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K(Ca)3.1 upregulation preserves endothelium-dependent vasorelaxation during aging and oxidative stress

Title
K(Ca)3.1 upregulation preserves endothelium-dependent vasorelaxation during aging and oxidative stress
Authors
Choi, ShinkyuKim, Ji AeeLi, Hai-yanShin, Kyong-OhOh, Goo TaegLee, Yong-MoonOh, SeikwanPewzner-Jung, YaelFuterman, Anthony H.Suh, Suk Hyo
Ewha Authors
서석효오세관오구택
SCOPUS Author ID
서석효scopus; 오세관scopus; 오구택scopus
Issue Date
2016
Journal Title
AGING CELL
ISSN
1474-9718JCR Link1474-9726JCR Link
Citation
vol. 15, no. 5, pp. 801 - 810
Keywords
agingCa2+-activated K+ channelceramide synthase 2 ablationendothelial cellsoxidative stressredox enzymes
Publisher
WILEY-BLACKWELL
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Endothelial oxidative stress develops with aging and reactive oxygen species impair endothelium-dependent relaxation (EDR) by decreasing nitric oxide (NO) availability. Endothelial K(Ca)3.1, which contributes to EDR, is upregulated by H2O2. We investigated whether K(Ca)3.1 upregulation compensates for diminished EDR to NO during aging-related oxidative stress. Previous studies identified that the levels of ceramide synthase 5 (CerS5), sphingosine, and sphingosine 1-phosphate were increased in aged wild-type and CerS2 mice. In primary mouse aortic endothelial cells (MAECs) from aged wild-type and CerS2 null mice, superoxide dismutase (SOD) was upregulated, and catalase and glutathione peroxidase 1 (GPX1) were downregulated, when compared to MAECs from young and age-matched wild-type mice. Increased H2O2 levels induced Fyn and extracellular signal-regulated kinases (ERKs) phosphorylation and K(Ca)3.1 upregulation. Catalase/GPX1 double knockout (catalase(-/-)/GPX1(-/-)) upregulated K(Ca)3.1 in MAECs. NO production was decreased in aged wild-type, CerS2 null, and catalase(-/-)/GPX1(-/-) MAECs. However, K(Ca)3.1 activation-induced, N-G-nitro-l-arginine-, and indomethacin-resistant EDR was increased without a change in acetylcholine-induced EDR in aortic rings from aged wild-type, CerS2 null, and catalase(-/-)/GPX1(-/-) mice. CerS5 transfection or exogenous application of sphingosine or sphingosine 1-phosphate induced similar changes in levels of the antioxidant enzymes and upregulated K(Ca)3.1. Our findings suggest that, during aging-related oxidative stress, SOD upregulation and downregulation of catalase and GPX1, which occur upon altering the sphingolipid composition or acyl chain length, generate H2O2 and thereby upregulate K(Ca)3.1 expression and function via a H2O2/Fyn-mediated pathway. Altogether, enhanced K(Ca)3.1 activity may compensate for decreased NO signaling during vascular aging.
DOI
10.1111/acel.12502
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의학전문대학원 > 의학과 > Journal papers
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