Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 문병인 | * |
dc.contributor.author | 오억수 | * |
dc.contributor.author | 정희성 | * |
dc.date.accessioned | 2016-08-29T12:08:02Z | - |
dc.date.available | 2016-08-29T12:08:02Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 0006-291X | * |
dc.identifier.issn | 1090-2104 | * |
dc.identifier.other | OAK-18998 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/231708 | - |
dc.description.abstract | E-cadherin plays a mechanical role in mediating cell-cell interactions and maintaining epithelial tissue integrity, and the loss of E-cadherin function has been implicated in cancer progression and metastasis. Syndecan-2, a cell-surface heparan sulfate proteoglycan, is upregulated during the development of colon cancer. Here, we assessed the functional relationship between E-cadherin and syndecan-2. We found that stable overexpression of syndecan-2 in a human colorectal adenocarcinoma cell line (HT29) enhanced the proteolytic shedding of E-cadherin to conditioned-media. Either knockdown of matrix metalloproteinase 7 (MMP-7) or inhibition of MMP-7 activity using GM6001 significantly reduced the extracellular shedding of E-cadherin, suggesting that syndecan-2 mediates E-cadherin shedding via MMP-7. Consistent with this notion, enhancement of MMP-7 expression by interleukin-1 alpha treatment increased the shedding of E-cadherin. Conversely, the specific reduction of either syndecan-2 or MMP-7 reduced the shedding of E-cadherin. HT29 cells overexpressing syndecan-2 showed significantly lower cell-surface expression of E-cadherin, decreased cell-cell contact, a more fibroblastic cell morphology, and increased expression levels of ZEB-1. Taken together, these data suggest that syndecan-2 induces extracellular shedding of E-cadherin and supports the acquisition of a fibroblast-like morphology by regulating MMP-7 expression in a colon cancer cell line. (C) 2016 Published by Elsevier Inc. | * |
dc.language | English | * |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | * |
dc.subject | E-cadherin | * |
dc.subject | E-cadherin shedding | * |
dc.subject | Syndecan-2 | * |
dc.subject | Matrix metalloprotease-7 | * |
dc.subject | Epithelial mesenchymal transition | * |
dc.subject | Colon cancer | * |
dc.title | Syndecan-2 enhances E-cadherin shedding and fibroblast-like morphological changes by inducing MMP-7 expression in colon cancer cells | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 477 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 47 | * |
dc.relation.lastpage | 53 | * |
dc.relation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | * |
dc.identifier.doi | 10.1016/j.bbrc.2016.06.019 | * |
dc.identifier.wosid | WOS:000380297000008 | * |
dc.identifier.scopusid | 2-s2.0-84976863875 | * |
dc.author.google | Jang, Bohee | * |
dc.author.google | Jung, Hyejung | * |
dc.author.google | Chung, Heesung | * |
dc.author.google | Moon, Byung-In | * |
dc.author.google | Oh, Eok-Soo | * |
dc.contributor.scopusid | 문병인(7101878644;56119062300) | * |
dc.contributor.scopusid | 오억수(7101967153) | * |
dc.contributor.scopusid | 정희성(35106799300) | * |
dc.date.modifydate | 20231116123537 | * |