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The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failure

Title
The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failure
Authors
Bae H.K.Lee H.Kim K.C.Hong Y.M.
Ewha Authors
홍영미김관창
SCOPUS Author ID
홍영미scopus; 김관창scopus
Issue Date
2016
Journal Title
Korean Journal of Pediatrics
ISSN
1738-1061JCR Link
Citation
vol. 59, no. 6, pp. 262 - 270
Keywords
Gene expressionPulmonary hypertensionSildenafil citrate
Publisher
Korean Pediatric Society
Indexed
SCOPUS; KCI scopus
Abstract
Purpose: Pulmonary arterial hypertension (PAH) leads to right ventricular failure (RVF) as well as an increase in pulmonary vascular resistance. Our purpose was to study the effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline (MCT)-induced RVF. Methods: The rats were distributed randomly into 3 groups. The control (C) group, the monocrotaline (M) group (MCT 60 mg/kg) and the sildenafil (S) group (MCT 60 mg/kg+ sildenafil 30 mg/kg/ day for 28 days). Masson Trichrome staining was used for heart tissues. Western blot analysis and immunohistochemical staining were performed. Results: The mean right ventricular pressure (RVP) was significantly lower in the S group at weeks 1, 2, and 4. The number of intra-acinar arteries and the medial wall thickness of the pulmonary arterioles significantly lessened in the S group at week 4. The collagen content also decreased in heart tissues in the S group at week 4. Protein expression levels of B-cell lymphoma-2 (Bcl-2)-associated X, caspase-3, Bcl-2, interleukin (IL)-6, matrix metalloproteinase (MMP)-2, endothelial nitric oxide synthase (eNOS), endothelin (ET)-1 and ET receptor A (ERA) in lung tissues greatly decreased in the S group at week 4 according to immunohistochemical staining. According to Western blotting, protein expression levels of troponin I, brain natriuretic peptide, caspase-3, Bcl-2, tumor necrosis factor-α, IL-6, MMP-2, eNOS, ET-1, and ERA in heart tissues greatly diminished in the S group at week 4. Conclusion: Sildenafil alleviated right ventricular hypertrophy and mean RVP. These data suggest that sildenafil improves right ventricular function. © 2016 by The Korean Pediatric Society.
DOI
10.3345/kjp.2016.59.5.262
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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