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FK506 positively regulates the migratory potential of melanocyte-derived cells by enhancing syndecan-2 expression

Title
FK506 positively regulates the migratory potential of melanocyte-derived cells by enhancing syndecan-2 expression
Authors
Jung, HyejungOh, Eok-Soo
Ewha Authors
오억수
SCOPUS Author ID
오억수scopus
Issue Date
2016
Journal Title
PIGMENT CELL & MELANOMA RESEARCH
ISSN
1755-1471JCR Link1755-148XJCR Link
Citation
vol. 29, no. 4, pp. 434 - 443
Keywords
vitiligomigrationFK506syndecan-2melanogenesis
Publisher
WILEY-BLACKWELL
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Although topical tacrolimus (FK506) is known to promote repigmentation by increasing the pigmentation and migration of melanocytes, the mechanism through which FK506 regulates cell migration remains unclear. Here, we report that FK506 treatment enhanced cell spreading on laminin-332 and increased migration in both melanocytes and melanoma cells. Interestingly, FK506 also increased the expression of syndecan-2, a transmembrane heparan sulfate proteoglycan through c-jun terminal kinase activation. Moreover, siRNA-mediated reduction of syndecan-2 expression decreased FK506-mediated cell spreading and migration in melanoma cells and decreased focal adhesion kinase phosphorylation in both melanocytes and melanoma cells. Consistent with these effects on syndecan-2 expression, FK506 enhanced the membrane and melanosome localizations of PKCbII, a regulator of tyrosinase activity. This suggests that FK506 may play a dual regulatory role by affecting both melanogenesis and migration in melanocyte-derived cells. Interestingly, however, FK506 failed to show any synergistic effect on the migration of UVB-treated melanocyte-derived cells. Taken together, these data indicate that FK506 regulates cell migration by enhancing syndecan-2 expression, further suggesting that syndecan-2 could be a potential target for the treatment of patients with vitiligo.
DOI
10.1111/pcmr.12480
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자연과학대학 > 생명과학전공 > Journal papers
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