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Necrotic cells influence migration and invasion of glioblastoma via NF-γ° B/AP-1-mediated IL-8 regulation

Title
Necrotic cells influence migration and invasion of glioblastoma via NF-γ° B/AP-1-mediated IL-8 regulation
Authors
Ahn S.-H.Park H.Ahn Y.-H.Kim S.Cho M.-S.Kang J.L.Choi Y.-H.
Ewha Authors
이지희조민선최윤희안영호
SCOPUS Author ID
이지희scopus; 조민선scopus; 최윤희scopus; 안영호scopus
Issue Date
2016
Journal Title
Scientific Reports
ISSN
2045-2322JCR Link
Citation
vol. 6
Publisher
Nature Publishing Group
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Glioblastoma multiforme (GBM) is the most common primary intracranial tumor in adults and has poor prognosis. Diffuse infiltration into normal brain parenchyma, rapid growth, and the presence of necrosis are remarkable hallmarks of GBM. However, the effect of necrotic cells on GBM growth and metastasis is poorly understood at present. In this study, we examined the biological significance of necrotic tissues by exploring the molecular mechanisms underlying the signaling network between necrotic tissues and GBM cells. The migration and invasion of the GBM cell line CRT-MG was significantly enhanced by treatment with necrotic cells, as shown by assays for scratch wound healing and spheroid invasion. Incubation with necrotic cells induced IL-8 secretion in CRT-MG cells in a dose-dependent manner. In human GBM tissues, IL-8 positive cells were mainly distributed in the perinecrotic region, as seen in immunohistochemistry and immunofluorescence analysis. Necrotic cells induced NF-κ B and AP-1 activation and their binding to the IL-8 promoter, leading to enhanced IL-8 production and secretion in GBM cells. Our data demonstrate that when GBM cells are exposed to and stimulated by necrotic cells, the migration and invasion of GBM cells are enhanced and facilitated via NF-κ B/AP-1 mediated IL-8 upregulation.
DOI
10.1038/srep24552
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의과대학 > 의학과 > Journal papers
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