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Expression of tenocyte lineage-related factors from tonsil-derived mesenchymal stem cells

Title
Expression of tenocyte lineage-related factors from tonsil-derived mesenchymal stem cells
Authors
Yu Y.Lee S.Y.Yang E.-J.Kim H.Y.Jo I.Shin S.-J.
Ewha Authors
신상진조인호유연실이승열
SCOPUS Author ID
신상진scopus; 조인호scopus; 유연실scopus; 이승열scopus
Issue Date
2016
Journal Title
Tissue Engineering and Regenerative Medicine
ISSN
1738-2696JCR Link
Citation
vol. 13, no. 2, pp. 162 - 170
Keywords
TenogenesisTonsil-derived mesenchymal stem cellsTransforming growth factor beta 3
Publisher
Kluwer Academic Publishers
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Human palatine tonsil-derived mesenchymal stem cells (TMSCs) are known to be a new source of progenitor cells. Using waste tissue after tonsillectomy as a cell provider can be the biggest benefit of TMSCs, compared with other stem cells. The purpose of this study was to investigate tenogenic differentiation of TMSCs and to access the differential effects of transforming growth factor beta 3 (TGF-β3) on the tenogenesis of TMSCs. Human tonsil was obtained after tonsillectomy. Using a cytometric analysis, we were able to find that the TMSCs had typical mesenchymal stem cell markers: positive for CD73, CD90, and CD105, and negative for CD14, CD34, and CD45. Using TGF-β3, the expressions of tenocyte-specific genes and proteins, such as collagen type 1 (COL1), tenomodulin (TNMD), and scleraxis (SCX), were measured by a quantitative polymerase chain reaction (PCR), immunofluorescence staining, immunohistochemistry and Western blot analyses. Quantitative PCR assay showed that TGF-β3 significantly increased the expressions of tenocyte lineage marker genes, including COL1, TNMD, and SCX, at a 3-day treatment, compared with control. However, these increases were not found at long-term exposures (7 or 10 days), except that TNMD expression was maintained at 50 ng/mL at a 7-day exposure to TGF-β3. Like genes, the protein expression levels of COL1, TNMD, and SCX were also induced in TGF-β3-treated TMSCs in a 3-day treatment, which were maintained for 10 days, as evidenced by immunofluorescence staining, immunohistochemistry and Western blot analyses. This study demonstrated that TMSCs in tenogenic stimulation with TGF-β3 have a high tenogenic differentiation potential. © 2016, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.
DOI
10.1007/s13770-016-9134-x
Appears in Collections:
의과대학 > 의학과 > Journal papers
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