Purpose: We investigated penile sensory levels in patients with primary premature ejaculation to determine whether there is an etiological basis for this condition. Materials and Methods: Penile biothesiometry was performed in 120 patients with primary premature ejaculation without erectile dysfunction and neurological deficit, and in 66 normal potent male volunteers. Vibratory thresholds were recorded at the glans penis, penile shaft, scrotum and index finger using a biothesiometer. Results: On the glans penis and penile shaft the values in patients with premature ejaculation were significantly less than those in normal potent men (p <0.001). In normal potent men an age dependency of biothesiometric parameters was noted, with a significant increase in vibratory threshold at the glans penis and penile shaft in older patients. However, in patients with premature ejaculation there were consistently decreased values without age dependency at the glans penis and penile shaft (p >0.05). Conclusions: Patients with primary premature ejaculation have penile hypersensitivity, which provides further implications for an organic basis of premature ejaculation.