Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 한종인 | * |
dc.contributor.author | 백희정 | * |
dc.contributor.author | 이희승 | * |
dc.contributor.author | 우재희 | * |
dc.date.accessioned | 2016-08-29T12:08:22Z | - |
dc.date.available | 2016-08-29T12:08:22Z | - |
dc.date.issued | 2012 | * |
dc.identifier.issn | 2005-6419 | * |
dc.identifier.other | OAK-18186 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/231287 | - |
dc.description.abstract | Background: Clonidine has been shown to be a potent neuroprotectant by acting at α 2 receptors on glutamatergicneurons to inhibit the release of glutamate. The aim of this study is to investigate the effects of clonidine on theactivity of EAAT3 that can regulate extracellular glutamate.Methods: EAAT3 was expressed in the Xenopus oocytes. Using a two-electrode voltage clamp, membrane currentswere recorded after application of 30 μM L-glutamate both in the presence and absence of various concentrationsof clonidine. To determine the effects of clonidine on the K m and Vmax of EAAT3 and the reversibility of clonidineeffects, membrane currents were recorded after the application of various concentrations of L-glutamate both in thepresence and absence of 1.50 × 10 -7 M clonidine. Results: Clonidine reduced the EAAT3 responses to L-glutamate in a concentration-dependent manner. Thisinhibition was statistically significant at higher concentrations than at the clinically relevant range. Clonidine at 1.50× 10 -7 M reduced the Vmax, but did not affect the K m of EAAT3 for L-glutamate.Conclusions: These results suggest that the direct inhibition of EAAT3 activity is not related to the sedation effectof clonidine and that the clonidine-induced reduction of EAAT3 activity provides additional data for the possibleinvolvement of glutamatergic hyperactivity in the proconvulsant effect of clonidine. © the Korean Society of Anesthesiologists, 2012. | * |
dc.language | English | * |
dc.title | Effects of clonidine on the activity of the rat glutamate transporter EAAT3 expressed in Xenopus oocytes | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 62 | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 266 | * |
dc.relation.lastpage | 271 | * |
dc.relation.journaltitle | Korean Journal of Anesthesiology | * |
dc.identifier.scopusid | 2-s2.0-84859148369 | * |
dc.author.google | Woo J.H. | * |
dc.author.google | Han J.I. | * |
dc.author.google | Baik H.J. | * |
dc.author.google | Lee H. | * |
dc.contributor.scopusid | 한종인(57206610922) | * |
dc.contributor.scopusid | 백희정(7101961242) | * |
dc.contributor.scopusid | 이희승(57216677572) | * |
dc.contributor.scopusid | 우재희(39162370700) | * |
dc.date.modifydate | 20240419142459 | * |