View : 21 Download: 0

Biodegradable Inorganic Nanovector: Passive versus Active Tumor Targeting in siRNA Transportation

Title
Biodegradable Inorganic Nanovector: Passive versus Active Tumor Targeting in siRNA Transportation
Authors
Park D.-H.Cho J.Kwon O.-J.Yun C.-O.Choy J.-H.
Ewha Authors
최진호
SCOPUS Author ID
최진호scopus
Issue Date
2016
Journal Title
Angewandte Chemie - International Edition
ISSN
1433-7851JCR Link
Keywords
Cancer therapyInorganic nanovectorsLayered compoundsNanoparticlesSiRNA delivery
Publisher
Wiley-VCH Verlag
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The biodegradable inorganic nanovector based on a layered double hydroxide (LDH) holds great promise for gene and drug delivery systems. However, invivo targeted delivery of genes through LDH still remains a key challenge in the development of RNA interference therapeutics. Here, we describe invivo and invitro delivery system for Survivin siRNA (siSurvivin) assembled with passive LDH with a particle size of 100nm or active LDH conjugated with a cancer overexpressing receptor targeting ligand, folic acid (LDHFA), conferring them an ability to target the tumor by either EPR-based clathrin-mediated or folate receptor-mediated endocytosis. When not only transfected into KB cells but also injected into xenograft mice, LDHFA/siSurvivin induced potent gene silencing at mRNA and protein levels invitro, and consequently achieved a 3.0-fold higher suppression of tumor volume than LDH/siSurvivin invivo. This anti-tumor effect was attributed to a selectively 1.2-fold higher accumulation of siSurvivin in tumor tissue compared with other organs. Targeting to the tumor with inorganic nanovector can guide and accelerate an evolution of next-generation theranosis system. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
DOI
10.1002/anie.201510844
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE