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AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells

Title
AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells
Authors
Kim N.Lee J.O.Lee H.J.Lee Y.W.Kim H.I.Kim S.J.Park S.H.Lee C.S.Ryoo S.W.Hwang G.-S.Kim H.S.
Ewha Authors
황금숙
SCOPUS Author ID
황금숙scopusscopus
Issue Date
2016
Journal Title
Journal of Endocrinology
ISSN
0022-0795JCR Link
Citation
vol. 228, no. 2, pp. 105 - 114
Keywords
AMPKGlucose uptakeIsoeugenolp38 MAPK
Publisher
BioScientifica Ltd.
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Isoeugenol exerts various beneficial effects on human health. However, the mechanisms underlying these effects are poorly understood. In this study, we observed that isoeugenol activated AMP-activated protein kinase (AMPK) and increased glucose uptake in rat L6 myotubes. Isoeugenol-induced increase in intracellular calcium concentration and glucose uptake was inhibited by STO-609, an inhibitor of calcium/calmodulin-dependent protein kinase kinase (CaMKK). Isoeugenol also increased the phosphorylation of protein kinase C-α (PKCα). Chelation of calcium with BAPTA-AM blocked isoeugenol-induced AMPK phosphorylation and glucose uptake. Isoeugenol stimulated p38MAPK phosphorylation that was inhibited after pretreatment with compound C, an AMPK inhibitor. Isoeugenol also increased glucose transporter type 4 (GLUT4) expression and its translocation to the plasma membrane. GLUT4 translocation was not observed after the inhibition of AMPK and CaMKK. In addition, isoeugenol activated the Akt substrate 160 (AS160) pathway, which is downstream of the p38MAPK pathway. Knockdown of the gene encoding AS160 inhibited isoeugenol-induced glucose uptake. Together, these results indicate that isoeugenol exerts beneficial health effects by activating the AMPK/p38MAPK/AS160 pathways in skeletal muscle. © 2016 The authors.
DOI
10.1530/JOE-15-0302
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자연과학대학 > 화학·나노과학전공 > Journal papers
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