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A lectin-based diagnostic system using circulating antibodies to detect cervical intraepithelial neoplasia and cervical cancer

Title
A lectin-based diagnostic system using circulating antibodies to detect cervical intraepithelial neoplasia and cervical cancer
Authors
Jin Y.Kim S.C.Kim H.J.Ju W.Kim Y.H.Kim H.-J.
Ewha Authors
주웅김윤환
SCOPUS Author ID
주웅scopus; 김윤환scopus
Issue Date
2015
Journal Title
Glycobiology
ISSN
0959-6658JCR Link
Citation
vol. 26, no. 1, pp. 100 - 107
Keywords
glycosylation and fucosylationimmunoglobulinlectinserum antibody
Publisher
Oxford University Press
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
In the present study, we developed serological strategies using immunoglobulin fractions obtained by protein A chromatography to screen for cervical cancer and cervical intraepithelial neoplasia I (CIN I). The reactivities of the immunoglobulins purified from sera of women with normal cytology, CIN I and cervical cancer were compared in enzyme-linked immunosorbent assays (ELISA) and enzyme-linked lectin assays (ELLAs). To capture the immunoglobulins, ELISAs and ELLAs were performed in protein A immobilized microplates. The reactivity of immunoglobulin in ELISA was in the increasing order normal cytology, CIN I and cervical cancer, while that in ELLAs for detecting fucosylation was in the decreasing order normal cytology, CIN I and cervical cancer. It was confirmed that women with CIN I were distinguishable from women with normal cytology or women with cervical cancer in the ELISA or the ELLA for detecting fucosylation with considerable sensitivity and specificity. Women with cervical cancer were also distinguishable from women with normal cytology with high sensitivity (ELISA: 97%, ELLA: 87%) and specificity (ELISA: 69%, ELLA: 72%). Moreover, the logistic regression model of the ELISA and the ELLA discriminated cervical cancer from normal cytology with 93% sensitivity and 93% specificity. These results indicate that the ELISAs and the ELLAs have great potential as strategies for primary screening of cervical cancer and CIN. It is expected that the ELISA and the ELLA can provide new insights to understand systemic changes of serum immunoglobulins during cervical cancer progression. © 2015 The Author. Published by Oxford University Press. All rights reserved.
DOI
10.1093/glycob/cwv075
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의학전문대학원 > 의학과 > Journal papers
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