View : 629 Download: 0
Protective effects of kaempferol against cardiac sinus node dysfunction via CaMKII deoxidization
- Title
- Protective effects of kaempferol against cardiac sinus node dysfunction via CaMKII deoxidization
- Authors
- An M.; Kim M.
- Ewha Authors
- 김민석
- SCOPUS Author ID
- 김민석
- Issue Date
- 2015
- Journal Title
- Anatomy and Cell Biology
- ISSN
- 2093-3665
- Citation
- Anatomy and Cell Biology vol. 48, no. 4, pp. 235 - 243
- Keywords
- Angiotensin II; CaMKII; Kaempferol; Sinus node dysfunction
- Publisher
- Korean Association of Anatomists
- Indexed
- SCOPUS; KCI
- Document Type
- Article
- Abstract
- Kaempferol exerts cardioprotective actions through incompletely understood mechanisms. This study investigated the molecular mechanisms underlying the cardioprotective effects of kaempferol in sinus node dysfunction (SND) heart. Here, we demonstrate that angiotensin II (Ang II) infusion causes SND through oxidized calmodulin kinase II (CaMKII). In contrast to this, kaempferol protects sinus node against Ang II-induced SND. Ang II evoked apoptosis with caspase-3 activation in sinus nodal cells. However, kaempferol lowered the CaMKII oxidization and the sinus nodal cell death. To block the CaMKII oxidization, gene of p47phox, a cytosolic subunit of NADPH oxidase, was deleted using Cas9 KO plasmid. In the absence of p47phox, sinus nodal cells were highly resistance to Ang II-induced apoptosis, suggesting that oxidized-CaMKII contributed to sinus nodal cell death. In Langendorff heart from Ang II infused mice, kaempferol preserved normal impulse formation at right atrium. These data suggested that kaempferol protects sinus node via inhibition of CaMKII oxidization and may be useful for preventing SND in high risk patients. © 2015.
- DOI
- 10.5115/acb.2015.48.4.235
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML