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Discrimination of skin sensitizers from non-sensitizers by interleukin-1 and interleukin-6 production on cultured human keratinocytes

Title
Discrimination of skin sensitizers from non-sensitizers by interleukin-1 and interleukin-6 production on cultured human keratinocytes
Authors
Jung, DaunChe, Jeong-HwanLim, Kyung-MinChun, Young-JinHeo, YongSeok, Seung Hyeok
Ewha Authors
임경민
SCOPUS Author ID
임경민scopus
Issue Date
2016
Journal Title
JOURNAL OF APPLIED TOXICOLOGY
ISSN
0260-437XJCR Link1099-1263JCR Link
Citation
vol. 36, no. 9, pp. 1129 - 1136
Keywords
cultured human keratinocyteHaCaTinterleukin-1interleukin-6skin sensitizer
Publisher
WILEY-BLACKWELL
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
In vitro testing methods for classifying sensitizers could be valuable alternatives to in vivo sensitization testing using animal models, such as the murine local lymph node assay (LLNA) and the guinea pig maximization test (GMT), but there remains a need for in vitro methods that are more accurate and simpler to distinguish skin sensitizers from non-sensitizers. Thus, the aim of our study was to establish an in vitro assay as a screening tool for detecting skin sensitizers using the human keratinocyte cell line, HaCaT. HaCaT cells were exposed to 16 relevant skin sensitizers and 6 skin non-sensitizers. The highest dose used was the dose causing 75% cell viability (CV75) that we determined by an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The levels of extracellular production of interleukin-1 (IL-1) and IL-6 were measured. The sensitivity of IL-1 was 63%, specificity was 83% and accuracy was 68%. In the case of IL-6, sensitivity: 69%, specificity: 83% and accuracy: 73%. Thus, this study suggests that measuring extracellular production of pro-inflammatory cytokines IL-1 and IL-6 by human HaCaT cells may potentially classify skin sensitizers from non-sensitizers. Copyright (c) 2015 John Wiley & Sons, Ltd. In vitro testing methods for classifying sensitizers could be valuable alternatives to in vivo sensitization testing using animal models, such as themurine local lymph node assay (LLNA) and the guinea pigmaximization test (GMT), but there remains a need for in vitro methods that are more accurate and simpler to distinguish skin sensitizers from non-sensitizers. Thus, the aim of our study was to establish an in vitro assay as a screening tool for detecting skin sensitizers using the human keratinocyte cell line, HaCaT.
DOI
10.1002/jat.3274
Appears in Collections:
약학대학 > 약학과 > Journal papers
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