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Fatty acid hydration activity of a recombinant Escherichia coli-based biocatalyst is improved through targeting the oleate hydratase into the periplasm

Title
Fatty acid hydration activity of a recombinant Escherichia coli-based biocatalyst is improved through targeting the oleate hydratase into the periplasm
Authors
Jung S.-M.Seo J.-H.Lee J.-H.Park J.-B.
Ewha Authors
박진병서주현
SCOPUS Author ID
박진병scopus; 서주현scopusscopus
Issue Date
2015
Journal Title
Biotechnology Journal
ISSN
1860-6768JCR Link
Citation
vol. 10, no. 12, pp. 1887 - 1893
Keywords
FAD-dependent oleate hydrataseFatty acidsPeriplasmic expressionWhole cell biotransformation
Publisher
Wiley-VCH Verlag
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Whole-cell biotransformation of fatty acids can be influenced by the activities of catalytic enzymes and by the efficiency of substrate transport into host cells. Here, we improved fatty acid hydration activity of the recombinant Escherichia coli expressing an oleate hydratase of Stenotrophomonas maltophilia by targeting the catalytic enzyme into the periplasm instead of the cytoplasm. Recombinant E. coli producing OhyA in the periplasm under guidance of the PelB signal sequence (E. coli OhyA_PP) exhibited significantly greater hydration activity with oleic acid and linoleic acid compared to a recombinant E. coli producing OhyA in the cytoplasm (E. coli OhyA_CS). For example, the oleate double bond hydration rate of E. coli OhyA_PP was >400 μmol/g dry cells/min (400 U/g dry cells), which is >10-fold higher than that of E. coli OhyA_CS. As the specific activities of the enzymes targeted into the cytoplasm and periplasm were comparable, we assumed that targeting OhyA into the periplasm could accelerate fatty acid transport to the catalytic enzymes by skipping the major mass transport barrier of the cytoplasmic membrane. Our results will contribute to the development of whole-cell biocatalysts for fatty acid biotransformation. Whole-cell biotransformation of fatty acids can be influenced by the activities of catalytic enzymes as well as the efficiency of substrate transport into host cells. In this work, the authors improve fatty acid hydration activity of the recombinant Escherichia coli which expresses an oleate hydratase of Stenotrophomonas maltophilia by increasing the efficiency of substrate transport into catalytic enzymes. This work will contribute to the development of whole-cell biocatalysts for fatty acid biotransformation. © 2015 WILEY-VCH Verlag GmbH & Co.
DOI
10.1002/biot.201500141
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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