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TIMP-1 affects the spatial distribution of dendritic processes of second-order neurons in a rat model of Retinitis Pigmentosa

Title
TIMP-1 affects the spatial distribution of dendritic processes of second-order neurons in a rat model of Retinitis Pigmentosa
Authors
Shin J.-A.Eom Y.S.Yu W.-Q.Grzywacz N.M.Craft C.M.Lee E.-J.
Ewha Authors
신정아
SCOPUS Author ID
신정아scopus
Issue Date
2015
Journal Title
Experimental Eye Research
ISSN
0014-4835JCR Link
Citation
vol. 140, pp. 41 - 52
Keywords
Bipolar cellCone mosaicCone outer segmentHorizontal cellRetinitis pigmentosaSynaptic proteinTissue inhibitor of metalloproteinase-1(TIMP-1)
Publisher
Academic Press
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Retinitis Pigmentosa (RP) is an inherited disorder that may lead to blindness. In the rhodopsin S334ter-line-3 rat model of RP, the death of rods induces spatial rearrangement of cones into regular ring mosaics. Using this model, we discovered that the ring mosaics are restored to a homogeneous distribution upon application of tissue inhibitor of metalloproteinase-1 (TIMP-1). In this study, we further investigated the cone migration and spatial distribution of second-order neurons and their connections to cones in the presence or absence of TIMP-1 using immunohistochemistry to identify retinal neurons and their connections with cones. M-opsin cell bodies and their outer segments were evaluated to determine whether TIMP-1 delays the degeneration of outer segments of cones. We observed that during cone rearrangement into ring mosaics in RP retina, dendritic processes of second-order neurons undergo remodeling to maintain their synaptic connections with the cones in the rings. TIMP-1 treatment induced the cones to rearrange and dendritic processes of second-order neurons to return to a more homogeneous spatial distribution. In addition, TIMP-1 treatment protected the outer segments of cones at later stages of retinal degeneration. Our findings clearly demonstrate that despite their dramatic spatial rearrangement, cones and second-order neuron processes maintain their synaptic connections before and after TIMP-1 treatment. © 2015 Elsevier Ltd.
DOI
10.1016/j.exer.2015.08.005
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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