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Synthetic approach to the generation of antibody diversity

Title
Synthetic approach to the generation of antibody diversity
Authors
ShimH.
Ewha Authors
심현보
SCOPUS Author ID
심현보scopus
Issue Date
2015
Journal Title
BMB Reports
ISSN
1976-6696JCR Link
Citation
vol. 48, no. 9, pp. 489 - 494
Keywords
Antibody libraryPhage displaySynthetic antibody diversity
Publisher
The Biochemical Society of the Republic of Korea
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
The in vitro antibody discovery technologies revolutionized the generation of target-specific antibodies that traditionally relied on the humoral response of immunized animals. An antibody library, a large collection of diverse, pre-constructed antibodies, can be rapidly screened using in vitro display technologies such as phage display. One of the keys to successful in vitro antibody discovery is the quality of the library diversity. Antibody diversity can be obtained either from natural B-cell sources or by the synthetic methods that combinatorially generate random nucleotide sequences. While the functionality of a natural antibody library depends largely upon the library size, various other factors can affect the quality of a synthetic antibody library, making the design and construction of synthetic antibody libraries complicated and challenging. In this review, we present various library designs and diversification methods for synthetic antibody library. From simple degenerate oligonucleotide synthesis to trinucleotide synthesis to physicochemically optimized library design, the synthetic approach is evolving beyond the simple emulation of natural antibodies, into a highly sophisticated method that is capable of producing high quality antibodies suitable for therapeutic, diagnostic, and other demanding applications. © 2015 by the The Korean Society for Biochemistry and Molecular Biology.
DOI
10.5483/BMBRep.2015.48.9.120
Appears in Collections:
일반대학원 > 바이오융합과학과 > Journal papers
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