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Comparison of the ocular surface changes following the use of two different prostaglandin F2a analogues containing benzalkonium chloride or polyquad in rabbit eyes

Title
Comparison of the ocular surface changes following the use of two different prostaglandin F2a analogues containing benzalkonium chloride or polyquad in rabbit eyes
Authors
Lee H.J.Jun R.M.Cho M.S.Choi K.-R.
Ewha Authors
최규룡조민선전루민
SCOPUS Author ID
최규룡scopus; 조민선scopus; 전루민scopus
Issue Date
2015
Journal Title
Cutaneous and Ocular Toxicology
ISSN
1556-9527JCR Link
Citation
vol. 34, no. 3, pp. 195 - 202
Keywords
Benzalkonium chlorideOcular surfacePolyquad
Publisher
Taylor and Francis Ltd
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Objective: The aim of this study is to compare the effect of prostaglandin analogues preserved with either 0.015% or 0.001% benzalkoium chloride (BAK); or 0.001% polyquad (PQ) on the ocular surface of rabbit eyes. Methods: Forty white rabbits were randomized to receive four-times daily instillation of either 0.0015% tafluprost (TF) preserved with 0.001% BAK (TF-BAK); 0.004% travoprost (TR) with 0.015% BAK (TR-BAK) or 0.001% PQ (TR-PQ); or preservative-free artificial tears in one eye for a 4-week period. Tear samples collected from the 40 rabbits were analyzed by enzyme-linked immunosorbent assays (ELISA) to identify the presence of inflammatory cytokines: interleukin (IL)-1b and IL-6 on day 14. Subsequently, harvested cornea and bulbar conjunctiva were evaluated using light and transmission electron microscopy (TEM). Results: IL-6 was significantly increased in TF-BAK and TR-BAK groups compared to controls and TR-PQ group (p=0.005); however, IL-1b level was not significantly different among four groups (p=0.360). Rabbits treated with TR-BAK showed decreased goblet cell density of bulbar conjunctiva and increased pyknotic change and vacuolization of corneal epithelial cells on light microscopy; similar change occurred but was less severe in TF-BAK group. The TR-PQ group showed similar results as the controls. The destruction of the microvillar architecture of bulbar conjunctiva and cornea was most prominent in the TR-BAK group. Conclusions: Preservatives included in the anti-glaucoma eye-drops showed different ocular surface changes according to the concentration and type in the rabbits. Prostaglandin analogues preserved with higher level of BAK may cause more harmful effects on the ocular surface than PQ-preserved medications.
DOI
10.3109/15569527.2014.944650
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의학전문대학원 > 의학과 > Journal papers
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