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Quality evaluation and pattern recognition analyses of bioactive marker compounds from Farfarae Flos using HPLC/PDA

Title
Quality evaluation and pattern recognition analyses of bioactive marker compounds from Farfarae Flos using HPLC/PDA
Authors
Seo U.M.Zhao B.T.Kim W.I.Seo E.K.Lee J.H.Min B.S.Shin B.S.Son J.K.Woo M.H.
Ewha Authors
서은경
SCOPUS Author ID
서은경scopus
Issue Date
2015
Journal Title
Chemical and Pharmaceutical Bulletin
ISSN
9236-2363JCR Link
Citation
vol. 63, no. 7, pp. 546 - 553
Keywords
Farfarae FlosHPLCPattern recognitionQuality control
Publisher
Pharmaceutical Society of Japan
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The flower bud of Tussilago farfara L., called Farfarae Flos, has traditionally been used in Oriental medicine for the treatment of bronchitis and asthma. To establish a standard for quality control as well as the reliable identification of Farfarae Flos, the contents of five standards, rutin (1), isoquercetin (2), 3,5-dicaffeoylquinic acid (3), tussilagone (4), and tussilagonone (5), were determined by quantitative high-performance liquid chromatography (HPLC)/photodiode array (PDA) analysis. The five standards were separated on a Young Jin Bio Chrom Aegispak C18-L (250-mm×4.6-mm, 5-μm) column by gradient elution using 0.03% trifluoroacetic acid in water (A), with acetonitrile (B) as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 220 nm. The method was successfully used in the analysis of Farfarae Flos from different geographic origins with relatively simple conditions and procedures, and the results demonstrated satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 62 Farfarae Flos samples. This result indicated that the established HPLC/PDA method is suitable for quantitation and pattern recognition analyses for the quality evaluation of Farfarae Flos. © 2015 The Pharmaceutical Society of Japan.
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약학대학 > 약학과 > Journal papers
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