Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이지수 | - |
dc.contributor.author | 김인제 | - |
dc.date.accessioned | 2016-08-29T12:08:20Z | - |
dc.date.available | 2016-08-29T12:08:20Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1478-6354 | - |
dc.identifier.other | OAK-15324 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230711 | - |
dc.description.abstract | Introduction: Pre-naïve B cells represent an intermediate stage in human B-cell development with some functions of mature cells, but their involvement in immune responses is unknown. The aim of this study was to determine the functional role of normal pre-naïve B cells during immune responses and possible abnormalities in systemic lupus erythematosus (SLE) that might contribute to disease pathogenesis. Methods: Pre-naïve, naïve, and memory B cells from healthy individuals and SLE patients were stimulated through CD40 and were analyzed for interleukin-10 (IL-10) production and co-stimulatory molecule expression and their regulation of T-cell activation. Autoreactivity of antibodies produced by pre-naïve B cells was tested by measuring immunoglobulin M (IgM) autoantibodies in culture supernatants after differentiation. Results: CD40-stimulated pre-naïve B cells produce larger amounts of IL-10 but did not suppress CD4+ T-cell cytokine production. Activated pre-naïve B cells demonstrated IL-10-mediated ineffective promotion of CD4+ T-cell proliferation and induction of CD4+FoxP3+ T cells and IL-10 independent impairment of co-stimulatory molecule expression and tumor necrosis factor-alpha (TNF-aα) and IL-6 production. IgM antibodies produced by differentiated pre-naïve B cells were reactive to single-stranded deoxyribonucleic acid. SLE pre-naïve B cells were defective in producing IL-10, and co-stimulatory molecule expression was enhanced, resulting in promotion of robust CD4+ T-cell proliferation. Conclusions: There is an inherent and IL-10-mediated mechanism that limits the capacity of normal pre-naïve B cells from participating in cellular immune response, but these cells can differentiate into autoantibody-secreting plasma cells. In SLE, defects in IL-10 secretion permit pre-naïve B cells to promote CD4+ T-cell activation and may thereby enhance the development of autoimmunity. © 2015 Sim et al. | - |
dc.language | English | - |
dc.publisher | BioMed Central Ltd. | - |
dc.title | Autoregulatory function of interleukin-10-producing pre-naïve B cells is defective in systemic lupus erythematosus | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 17 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.journaltitle | Arthritis Research and Therapy | - |
dc.identifier.doi | 10.1186/s13075-015-0687-1 | - |
dc.identifier.wosid | WOS:000358400800002 | - |
dc.identifier.scopusid | 2-s2.0-84938291830 | - |
dc.author.google | Sim J.H. | - |
dc.author.google | Kim H.-R. | - |
dc.author.google | Chang S.-H. | - |
dc.author.google | Kim I.J. | - |
dc.author.google | Lipsky P.E. | - |
dc.author.google | Lee J. | - |
dc.contributor.scopusid | 이지수(14424388700) | - |
dc.contributor.scopusid | 김인제(55477686200;56596890100) | - |
dc.date.modifydate | 20230201112642 | - |