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dc.contributor.author이지수-
dc.contributor.author김인제-
dc.date.accessioned2017-08-29T04:06:13Z-
dc.date.available2017-08-29T04:06:13Z-
dc.date.issued2015-
dc.identifier.issn1478-6354-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/230711-
dc.description.abstractIntroduction: Pre-naïve B cells represent an intermediate stage in human B-cell development with some functions of mature cells, but their involvement in immune responses is unknown. The aim of this study was to determine the functional role of normal pre-naïve B cells during immune responses and possible abnormalities in systemic lupus erythematosus (SLE) that might contribute to disease pathogenesis. Methods: Pre-naïve, naïve, and memory B cells from healthy individuals and SLE patients were stimulated through CD40 and were analyzed for interleukin-10 (IL-10) production and co-stimulatory molecule expression and their regulation of T-cell activation. Autoreactivity of antibodies produced by pre-naïve B cells was tested by measuring immunoglobulin M (IgM) autoantibodies in culture supernatants after differentiation. Results: CD40-stimulated pre-naïve B cells produce larger amounts of IL-10 but did not suppress CD4+ T-cell cytokine production. Activated pre-naïve B cells demonstrated IL-10-mediated ineffective promotion of CD4+ T-cell proliferation and induction of CD4+FoxP3+ T cells and IL-10 independent impairment of co-stimulatory molecule expression and tumor necrosis factor-alpha (TNF-aα) and IL-6 production. IgM antibodies produced by differentiated pre-naïve B cells were reactive to single-stranded deoxyribonucleic acid. SLE pre-naïve B cells were defective in producing IL-10, and co-stimulatory molecule expression was enhanced, resulting in promotion of robust CD4+ T-cell proliferation. Conclusions: There is an inherent and IL-10-mediated mechanism that limits the capacity of normal pre-naïve B cells from participating in cellular immune response, but these cells can differentiate into autoantibody-secreting plasma cells. In SLE, defects in IL-10 secretion permit pre-naïve B cells to promote CD4+ T-cell activation and may thereby enhance the development of autoimmunity. © 2015 Sim et al.-
dc.languageEnglish-
dc.publisherBioMed Central Ltd.-
dc.titleAutoregulatory function of interleukin-10-producing pre-naïve B cells is defective in systemic lupus erythematosus-
dc.typeArticle-
dc.relation.issue1-
dc.relation.volume17-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.journaltitleArthritis Research and Therapy-
dc.identifier.doi10.1186/s13075-015-0687-1-
dc.identifier.wosidWOS:000358400800002-
dc.identifier.scopusid2-s2.0-84938291830-
dc.author.googleSim J.H.-
dc.author.googleKim H.-R.-
dc.author.googleChang S.-H.-
dc.author.googleKim I.J.-
dc.author.googleLipsky P.E.-
dc.author.googleLee J.-
dc.contributor.scopusid이지수(14424388700)-
dc.contributor.scopusid김인제(55477686200)-
dc.date.modifydate20180329110821-


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