Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 한평림 | - |
dc.date.accessioned | 2016-08-29T12:08:55Z | - |
dc.date.available | 2016-08-29T12:08:55Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1528-3658 | - |
dc.identifier.other | OAK-14719 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230484 | - |
dc.description.abstract | Ethyl pyruvate (EP), a simple aliphatic ester of pyruvic acid, has been shown to have antiinflammatory effects and to confer protective effects in various pathological conditions. Recently, a number of studies have reported EP inhibits high mobility group box 1 (HMGB1) secretion and suggest this might contribute to its antiinflammatory effect. Since EP is used in a calcium-containing balanced salt solution (Ringer solution), we wondered if EP directly chelates Ca(2+) and if it is related to the EP-mediated suppression of HMGB1 release. Calcium imaging assays revealed that EP significantly and dose-dependently suppressed high K(+)-induced transient [Ca(2+)]i surges in primary cortical neurons and, similarly, fluorometric assays showed that EP directly scavenges Ca(2+) as the peak of fluorescence emission intensities of Mag-Fura-2 (a low-affinity Ca(2+) indicator) was shifted in the presence of EP at concentrations of ≥7 mmol/L. Furthermore, EP markedly suppressed the A23187-induced intracellular Ca(2+) surge in BV2 cells and, under this condition, A23187-induced activations of Ca(2+)-mediated kinases (protein kinase Cα and calcium/calmodulin-dependent protein kinase IV), HMGB1 phosphorylation and subsequent secretion of HMGB1 also were suppressed. (A23187 is a calcium ionophore and BV2 cells are a microglia cell line.) Moreover, the above-mentioned EP-mediated effects were obtained independent of cell death or survival, which suggests that they are direct effects of EP. Together, these results indicate that EP directly chelates Ca(2+), and that it is, at least in part, responsible for the suppression of HMGB1 release by EP. | - |
dc.language | English | - |
dc.title | Ethyl pyruvate inhibits HMGB1 phosphorylation and release by chelating calcium | - |
dc.type | Article | - |
dc.relation.volume | 20 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 649 | - |
dc.relation.lastpage | 657 | - |
dc.relation.journaltitle | Molecular medicine (Cambridge, Mass.) | - |
dc.identifier.doi | 10.2119/molmed.2014.00039 | - |
dc.identifier.wosid | WOS:000351749900004 | - |
dc.identifier.scopusid | 2-s2.0-84965089222 | - |
dc.author.google | Shin J.-H. | - |
dc.author.google | Kim I.-D. | - |
dc.author.google | Kim S.-W. | - |
dc.author.google | Lee H.-K. | - |
dc.author.google | Jin Y. | - |
dc.author.google | Park J.-H. | - |
dc.author.google | Kim T.-K. | - |
dc.author.google | Suh C.-K. | - |
dc.author.google | Kwak J. | - |
dc.author.google | Lee K.-H. | - |
dc.author.google | Han P.-L. | - |
dc.author.google | Lee J.-K. | - |
dc.contributor.scopusid | 한평림(7201947605) | - |
dc.date.modifydate | 20230901081001 | - |