Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조인호 | * |
dc.date.accessioned | 2016-08-29T12:08:54Z | - |
dc.date.available | 2016-08-29T12:08:54Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0567-8315 | * |
dc.identifier.other | OAK-14693 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230474 | - |
dc.description.abstract | A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100 L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples. | * |
dc.language | English | * |
dc.publisher | Walter de Gruyter GmbH | * |
dc.subject | Lawsonia intracellularis | * |
dc.subject | Pig | * |
dc.subject | Porcine proliferative enteropathy | * |
dc.subject | Real-time loopmediated isothermal amplification | * |
dc.title | Rapid and sensitive detection of Lawsonia intracellularis in pigs by real-time loop-mediated isothermal amplification | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 65 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 20 | * |
dc.relation.lastpage | 29 | * |
dc.relation.journaltitle | Acta Veterinaria | * |
dc.identifier.doi | 10.1515/acve-2015-0002 | * |
dc.identifier.wosid | WOS:000351737400002 | * |
dc.identifier.scopusid | 2-s2.0-84927634884 | * |
dc.author.google | Park B.-Y. | * |
dc.author.google | Shim K.-S. | * |
dc.author.google | Kim W.-I. | * |
dc.author.google | Hossain Md.M. | * |
dc.author.google | Kim B. | * |
dc.author.google | Kwon J. | * |
dc.author.google | Park C.-K. | * |
dc.author.google | Cho S.-J. | * |
dc.author.google | Jo I. | * |
dc.author.google | Cho H.-S. | * |
dc.contributor.scopusid | 조인호(26643129000;56663841900) | * |
dc.date.modifydate | 20240123112949 | * |