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Rapid and sensitive detection of Lawsonia intracellularis in pigs by real-time loop-mediated isothermal amplification

Title
Rapid and sensitive detection of Lawsonia intracellularis in pigs by real-time loop-mediated isothermal amplification
Authors
Park B.-Y.Shim K.-S.Kim W.-I.Hossain Md.M.Kim B.Kwon J.Park C.-K.Cho S.-J.Jo I.Cho H.-S.
Ewha Authors
조인호
SCOPUS Author ID
조인호scopus
Issue Date
2015
Journal Title
Acta Veterinaria
ISSN
0567-8315JCR Link
Citation
vol. 65, no. 1, pp. 20 - 29
Keywords
Lawsonia intracellularisPigPorcine proliferative enteropathyReal-time loopmediated isothermal amplification
Publisher
Walter de Gruyter GmbH
Indexed
SCIE; SCOPUS WOS scopus
Abstract
A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100 L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples.
DOI
10.1515/acve-2015-0002
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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