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Redox-active protein Thioredoxin-1 administration ameliorates influenza a virus (H1N1)-induced acute lung injury in mice

Title
Redox-active protein Thioredoxin-1 administration ameliorates influenza a virus (H1N1)-induced acute lung injury in mice
Authors
Yashiro M.Tsukahara H.Matsukawa A.Yamada M.Fujii Y.Nagaoka Y.Tsuge M.Yamashita N.Ito T.Masutani H.Yodoi J.Morishima T.
Ewha Authors
Junji Yodoi
Issue Date
2013
Journal Title
Critical Care Medicine
ISSN
0090-3493JCR Link
Citation
Critical Care Medicine vol. 41, no. 1, pp. 171 - 181
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
OBJECTIVES:: Influenza virus infections can cause severe acute lung injury leading to significant morbidity and mortality. Thioredoxin-1 is a redox-active defensive protein induced in response to stress conditions. Animal experiments have revealed that thioredoxin-1 has protective effects against various severe disorders. This study was undertaken to evaluate the protective effects of recombinant human thioredoxin-1 administration on influenza A virus (H1N1)-induced acute lung injury in mice. DESIGN:: Prospective animal trial. SETTING:: Research laboratory. SUBJECTS:: Nine-week-old male C57BL/6 mice inoculated with H1N1. INTERVENTION:: The mice were divided into a vehicle-treated group and recombinant human thioredoxin-1-treated group. For survival rate analysis, the vehicle or recombinant human thioredoxin-1 was administered intraperitoneally every second day from day-1 to day 13. For lung lavage and pathological analyses, vehicle or recombinant human thioredoxin-1 was administered intraperitoneally on days-1, 1, and 3. MEASUREMENTS AND MAIN RESULTS:: Lung lavage and pathological analyses were performed at 24, 72, and 120 hrs after inoculation. The recombinant human thioredoxin-1 treatment significantly improved the survival rate of H1N1-inoculated mice, although the treatment did not affect virus propagation in the lung. The treatment significantly attenuated the histological changes and neutrophil infiltration in the lung of H1N1-inoculated mice. The treatment significantly attenuated the production of tumor necrosis factor-α and chemokine (C-X-C motif) ligand 1 in the lung and oxidative stress enhancement, which were observed in H1N1-inoculated mice. H1N1 induced expressions of tumor necrosis factor-α and chemokine (C-X-C motif) ligand 1 in murine lung epithelial cells MLE-12, which were inhibited by the addition of recombinant human thioredoxin-1. The recombinant human thioredoxin-1 treatment started 30 mins after H1N1 inoculation also significantly improved the survival of the mice. CONCLUSIONS:: Exogenous administration of recombinant human thioredoxin-1 significantly improved the survival rate and attenuated lung histological changes in the murine model of influenza pneumonia. The protective mechanism of thioredoxin-1 might be explained by its potent antioxidative and anti-inflammatory actions. Consequently, recombinant human thioredoxin-1 might be a possible pharmacological strategy for severe influenza virus infection in humans. © 2013 by the Society of Critical Care Medicine and Lippincott Williams & Wilkins.
DOI
10.1097/CCM.0b013e3182676352
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일반대학원 > 바이오융합과학과 > Journal papers
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