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dc.contributor.author전길자-
dc.contributor.author배윤수-
dc.contributor.author한소엽-
dc.contributor.author김재상-
dc.contributor.author임진경-
dc.date.accessioned2017-08-29T03:06:22Z-
dc.date.available2017-08-29T03:06:22Z-
dc.date.issued2012-
dc.identifier.issn1932-6254-
dc.identifier.otherOAK-13871-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/229815-
dc.description.abstractIn this study we showed that 2-(trimethylammonium)ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate [(R)-TEMOSPho], a derivative of an organic chemical identified from a natural product library, promotes highly efficient differentiation of megakaryocytes. Specifically, (R)-TEMOSPho induces cell cycle arrest, cell size increase and polyploidization from K562 and HEL cells, which are used extensively to model megakaryocytic differentiation. In addition, megakaryocyte-specific cell surface markers showed a dramatic increase in expression in response to (R)-TEMOSPho treatment. Importantly, we demonstrated that such megakaryocytic differentiation can also be induced from primary human CD34 + haematopoietic stem cells. Activation of the PI3K-AKT pathway and, to a lesser extent, the MEK-ERK pathway appears to be required for this process, as blocking with specific inhibitors interferes with the differentiation of K562 cells. A subset of (R)-TEMOSPho-treated K562 cells undergoes spontaneous apoptosis and produces platelets that are apparently functional, as they bind to fibrinogen, express P-selectin and aggregate in response to SFLLRN and AYPGFK, the activating peptides for the PAR1 and PAR4 receptors, respectively. Taken together, these results indicate that (R)-TEMOSPho will be useful for dissecting the molecular mechanisms of megakaryocytic differentiation, and that this class of compounds represents potential therapeutic reagents for thrombocytopenia. © 2012 John Wiley & Sons, Ltd.-
dc.languageEnglish-
dc.title2-(Trimethylammonium)ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate promotes megakaryocytic differentiation of myeloid leukaemia cells and primary human CD34 + haematopoietic stem cells-
dc.typeArticle in Press-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.journaltitleJournal of Tissue Engineering and Regenerative Medicine-
dc.identifier.doi10.1002/term.1628-
dc.identifier.wosidWOS:000353034100011-
dc.identifier.scopusid2-s2.0-84869203990-
dc.author.googleLimb J.-K.-
dc.author.googleSong D.-
dc.author.googleJeon M.-
dc.author.googleHan S.-Y.-
dc.author.googleHan G.-
dc.author.googleJhon G.-J.-
dc.author.googleBae Y.S.-
dc.author.googleKim J.-
dc.contributor.scopusid전길자(6701488476)-
dc.contributor.scopusid배윤수(15031067200)-
dc.contributor.scopusid한소엽(7405944194)-
dc.contributor.scopusid김재상(8643335800)-
dc.contributor.scopusid임진경(7003530407)-
dc.date.modifydate20191108081000-
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자연과학대학 > 화학·나노과학전공 > Journal papers
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