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Sustained delivery of PTD-DNA from polymeric scaffold into adipose-derived stem cells

Title
Sustained delivery of PTD-DNA from polymeric scaffold into adipose-derived stem cells
Authors
Jung M.Kim J.Kim M.Park K.Lee S.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2008
Journal Title
8th World Biomaterials Congress 2008
Indexed
SCOPUS scopus
Abstract
We investigated an emulsion coated fibrous scaffold as a vehicle for delivery of DNA. PLGA emulsions containing EGFP-encoding plasmid were coated to PLLA fibers. DNA released from the fibers over time retained their bioactivity and successfully transferred into human adipose-derived stem cells (hADSCs) seeded on the scaffold in a sustained manner. DNA encoding EGFP fused to the MMG protein transduction domain (PTD) was efficiently transferred into hADSCs. Transgene expression in hADSCs on the DNA emulsion coated scaffolds was significant even after 2 weeks of culture compared to hADSCs treated DNA bolus. hADSCs seeded on scaffolds coated with emulsions containing EGFP plasmid demonstrated that continuous release of DNA offer extended gene expression and prolonged secretion of useful protein.
ISBN
9781615670802
Appears in Collections:
약학대학 > 약학과 > Journal papers
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