View : 11 Download: 9

A model of disturbed flow-induced atherosclerosis in mouse carotid artery by partial ligation and a simple method of rna isolation from carotid endothelium

Title
A model of disturbed flow-induced atherosclerosis in mouse carotid artery by partial ligation and a simple method of rna isolation from carotid endothelium
Authors
Nam D.Ni C.-W.Rezvan A.Suo J.Budzyn K.Llanos A.Harrison D.G.Giddens D.P.Jo H.
Ewha Authors
조한중
Issue Date
2010
Journal Title
Journal of Visualized Experiments
ISSN
1940-087XJCR Link
Citation
no. 40
Indexed
SCIE; SCOPUS scopus
Abstract
Despite the well-known close association, direct evidence linking disturbed flow to atherogenesis has been lacking. We have recently used a modified version of carotid partial ligation methods [1,2] to show that it acutely induces low and oscillatory flow conditions, two key characteristics of disturbed flow, in the mouse common carotid artery. Using this model, we have provided direct evidence that disturbed flow indeed leads to rapid and robust atherosclerosis development in Apolipoprotein E knockout mouse [3]. We also developed a method of endothelial RNA preparation with high purity from the mouse carotid intima [3]. Using this mouse model and method, we found that partial ligation causes endothelial dysfunction in a week, followed by robust and rapid atheroma formation in two weeks in a hyperlipidemic mouse model along with features of complex lesion formation such as intraplaque neovascularization by four weeks. This rapidin vivomodel and the endothelial RNA preparation method could be used to determine molecular mechanisms underlying flow-dependent regulation of vascular biology and diseases. Also, it could be used to test various therapeutic interventions targeting endothelial dysfunction and atherosclerosis in considerably reduced study duration. © JoVE 2006-2011 All Rights Reserved.
DOI
10.3791/1861
Appears in Collections:
일반대학원 > 바이오융합과학과 > Journal papers
Files in This Item:
model of disturbes.pdf(55.64 kB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE