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Gene expression of endothelin-1 and endothelin receptor A on monocrotaline-induced pulmonary hypertension in rats after bosentan treatment

Title
Gene expression of endothelin-1 and endothelin receptor A on monocrotaline-induced pulmonary hypertension in rats after bosentan treatment
Authors
Lim K.A.Kim K.C.Cho M.-S.Lee B.E.Kim H.S.Hong Y.M.
Ewha Authors
홍영미김혜순조민선김관창
SCOPUS Author ID
홍영미scopus; 김혜순scopus; 조민선scopus; 김관창scopus
Issue Date
2010
Journal Title
Korean Circulation Journal
ISSN
1738-5520JCR Link
Citation
vol. 40, no. 9, pp. 459 - 464
Indexed
SCIE; SCOPUS; KCI scopus
Abstract
Background and Objectives: Endothelin (ET)-1, a potent endothelium-derived vasoconstrictor peptide, has a potential pathophysiologic role in pulmonary hypertension. Bosentan, a dual ET receptor (ET A/ET B) antagonist, is efficacious in treatment of pulmonary hypertension. The objectives of this study were to investigate the expression of ET-1 and ET receptor A (ERA) genes and to evaluate the effect of bosentan in monocrotaline (MCT)-induced pulmonary hypertension. Materials and Methods: Four-week-old male Sprague-Dawley rats were treated as follows: control (n=36), subcutaneous (sc) injection of saline; MCT (n=36), sc injection of MCT (60 mg/kg); and bosentan (n=36), sc injection of MCT (60 mg/kg) plus 25 mg/kg/ day bosentan orally. Results: Serum ET-1 concentrations in the MCT group were higher than the control group on day 28 and 42. Quantitative analysis of peripheral pulmonary arteries revealed that the increase in medial wall thickness after MCT injection was significantly attenuated in the bosentan group on day 28 and 42. In addition, the increase in the number of intra-acinar muscular arteries after MCT injection was reduced by bosentan on day 14,28 and 42. The levels of ET-1 and ERA gene expression were significantly increased in the MCT group compared with control group on day 5, and bosentan decreased the expression of ET-1 on day 5. Conclusion: ET-1 contributes to the progression of cardiopulmonary pathology in rats with MCT-induced pulmonary hypertension. Administration of bosentan reduced ET-1 gene expression in MCT-induced pulmonary hypertension in rats. Copyright © 2010 The Korean Society of Cardiology.
DOI
10.4070/kcj.2010.40.9.459
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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