Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 명기범 | * |
dc.contributor.author | 최혜영 | * |
dc.contributor.author | 최유원 | * |
dc.contributor.author | 변지연 | * |
dc.date.accessioned | 2016-08-28T11:08:10Z | - |
dc.date.available | 2016-08-28T11:08:10Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 1013-9087 | * |
dc.identifier.other | OAK-13350 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/229360 | - |
dc.description.abstract | Background: Depending on the light dose and concentration of photosensitizer for photodynamic treatment (PDT), a multitude of dose-related events are demonstrable in PDT-treated cells. Sublethal doses may result in the alteration of cytokine release and consequently modify immune actions, rather than cause cell death. Objective: The purpose of this study was to investigate cytokine expression in cultured HaCaT cells after intense pulse light (IPL) treatment or PDT utilizing 5-aminolevulinic acid (ALA) and IPL at sublethal doses. Methods: Cultured HaCaT cells were treated with either IPL only (4, 8 and 12 J/cm2) or ALA-IPL PDT (100 μ mol/L of ALA; 0, 4, 8, and 12 J/cm2 of IPL). The expression of IL-10, TGF- β1 and TNF-α was investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. Results: IL-10 protein increased up to 5.95-fold after IPL treatment and up to 2.85-fold after PDT. TGF-β1 mRNA and protein showed slight increases after both IPL treatment and PDT, of which the latter induced slightly larger increases. TNF-α mRNA and protein showed no induction or reduction after PDT. Conclusion: Increased expressions of IL-10 and TGF-β1 was observed after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses, and that of TGF- β1 may be related to the therapeutic effect for psoriasis. The finding that IL-10 induction was more marked after IPL treatment than after PDT suggests that other mechanisms than IL-10 induction in keratinocytes after PDT may participate in the anti-inflammatory effect of PDT. (Ann Dermatol (Seoul) 21(1)12∼17,2009). | * |
dc.language | English | * |
dc.title | Expression of IL-10, TGF-β and TNF-α in cultured keratinocytes (HaCaT Cells) after IPL treatment or ALA-IPL photodynamic treatment | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 21 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 12 | * |
dc.relation.lastpage | 17 | * |
dc.relation.journaltitle | Annals of Dermatology | * |
dc.identifier.doi | 10.5021/ad.2009.21.1.12 | * |
dc.identifier.wosid | WOS:000268607800003 | * |
dc.identifier.scopusid | 2-s2.0-73249142558 | * |
dc.author.google | Byun J.Y. | * |
dc.author.google | Choi H.Y. | * |
dc.author.google | Myung K.B. | * |
dc.author.google | Choi Y.W. | * |
dc.contributor.scopusid | 명기범(7006220296) | * |
dc.contributor.scopusid | 최혜영(55724363200) | * |
dc.contributor.scopusid | 최유원(7404777738) | * |
dc.contributor.scopusid | 변지연(15724462400) | * |
dc.date.modifydate | 20240222131101 | * |