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Dihydroceramide was highly elevated by the fumonisin B1 and desipramine in Sphingomonas chungbukensis

Title
Dihydroceramide was highly elevated by the fumonisin B1 and desipramine in Sphingomonas chungbukensis
Authors
Burenjargal M.Lee Y.-S.Yoo J.-M.Choi M.-H.Ji S.-Y.Lee Y.-M.Kim Y.-C.Oh S.Yun Y.-P.Yoo H.-S.
Ewha Authors
오세관
SCOPUS Author ID
오세관scopus
Issue Date
2008
Journal Title
Biomolecules and Therapeutics
ISSN
1976-9148JCR Link
Citation
vol. 16, no. 2, pp. 100 - 105
Indexed
SCIE; SCOPUS WOS scopus
Abstract
The sphingolipid metabolites act as lipid mediator for cell proliferation and apoptosis in mammalian cells. In bacteria, sphingolipid metabolism remains unknown. The purpose of this study was to investigate whether sphingolipid metabolism is potential target for fumonisin B1(FB1) and desipramine in Sphingomonas chungbukensis, Gram-negative bacteria, by comparing the intracellular contents of bacterial sphingolipids with ones of HIT-T15 β-cells, hamster pancreatic cells. The concentrations of ceramide and dihydroceramide were 18.0 ± 12.0 and 0.025 ± 0.018 nmol/mg protein, respectively, in HIT-T15 cells. However, the concentrations of ceramide and dihydroceramide in the bacterial culture were 2.0 ± 1.2 and 10.6 ± 5.5 nmol/mg protein, respectively. FB1 decreased the level of ceramide from 18.0 to 3.8 nmol/mg protein in HIT-T15 β-cells. However, dihydroceramide content in FB1-treated HIT-T15 cells was slightly decreased compared with the control culture. When S. chungbukensis was treated with either FB1 or desipramine, dihydroceramide level was increased by 5- and 4-fold, respectively, compared with the control bacteria. These results indicate that FB1 and desipramine may act as an activator in bacterial sphingolipid biosynthetic pathway, and bacterial sphingolipid metabolism pathway appears to be different from the pathway of mammalian cells.
DOI
10.4062/biomolther.2008.16.2.100
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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