Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최수승 | * |
dc.contributor.author | 서석효 | * |
dc.date.accessioned | 2016-08-28T11:08:56Z | - |
dc.date.available | 2016-08-28T11:08:56Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 1226-4512 | * |
dc.identifier.other | OAK-13199 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/229228 | - |
dc.description.abstract | The effects of oxidized low-density lipoprotein (OxLDL) and its major lipid constituent lysophosphatidylcholine (LPC) on Ca2+ entry were investigated in cultured human umbilical endothelial cells (HUVECs) using fura-2 fluorescence and patch-clamp methods. OxLDL or LPC increased intracellular Ca2+ concentration ([Ca2+]i), and the increase of [Ca2+]i by OxLDL or by LPC was inhibited by La3+ or heparin. LPC failed to increase [Ca2+]i in the presence of an antioxidant tempol. In addition, store-operated Ca2+ entry (SOC), which was evoked by intracellular Ca2+ store depletion in Ca2+-free solution using the sarcoplasmic reticulum Ca2+ pump blocker, 2, 5-di-t-butyl-1, 4-benzohydroquinone (BHQY), was further enhanced by OxLDL or by LPC. Increased SOC by OxLDL or by LPC was inhibited by U73122. In voltage-clamped cells, OxLDL or LPC increased [Ca2+]i and simultaneously activated non-selective cation (NSC) currents. LPC-induced NSC currents were inhibited by 2-APB, La3+ or U73122, and NSC currents were not activated by LPC in the presence of tempol. Furthermore, in voltage-clamped HUVECs, OxLDL enhanced SOC and evoked outward currents simultaneously. Clampinig intracellular Ca2+ to 1 υM activated large-conductance Ca2+-activated K+ (BKCa) clirrent spontaneously, and this activated BKCa current was further enhanced bv OxLDL or by LPC. From these results, we concluded that OxLDL or its main component LPC activates Ca2+-permeable Ca2+-activated NSC current and BKCa current simultaneously, thereby increasing SOC. | * |
dc.language | English | * |
dc.title | Oxidized low-density lipoprotein- and lysophostidylcholine-induced Ca2+ mobilization in human endothelial cells | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 13 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 27 | * |
dc.relation.lastpage | 32 | * |
dc.relation.journaltitle | Korean Journal of Physiology and Pharmacology | * |
dc.identifier.doi | 10.4196/kjpp.2009.13.1.27 | * |
dc.identifier.wosid | WOS:000264174000005 | * |
dc.identifier.scopusid | 2-s2.0-62649136574 | * |
dc.author.google | Kim M.Y. | * |
dc.author.google | Liang G.H. | * |
dc.author.google | Kim J.A. | * |
dc.author.google | Choi S.S. | * |
dc.author.google | Choi S. | * |
dc.author.google | Suh S.H. | * |
dc.contributor.scopusid | 최수승(34869557900;56124295900;8707428200) | * |
dc.contributor.scopusid | 서석효(55666113100) | * |
dc.date.modifydate | 20240601081000 | * |