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Plasmodium falciparum cultivation using the Petri dish: Revisiting the effect of the 'age'of erythrocytes and the interval of medium change

Title
Plasmodium falciparum cultivation using the Petri dish: Revisiting the effect of the 'age'of erythrocytes and the interval of medium change
Authors
Kim Y.-A.Cha J.-E.Ahn S.-Y.Ryu S.-H.Yeom J.-S.Lee H.-I.Kim C.-G.Seoh J.-Y.Park J.-W.
Ewha Authors
서주영
SCOPUS Author ID
서주영scopus
Issue Date
2007
Journal Title
Journal of Korean Medical Science
ISSN
1011-8934JCR Link
Citation
vol. 22, no. 6, pp. 1022 - 1025
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Differences in the characteristics of the culture conditions can influence the multiplication rate of Plasmodium falciparum. The Petri dish method is one of the most popular methods of cultivating this parasite. In many previous studies, ideal culture conditions of the Petri dish method were achieved by using erythrocytes collected from blood that had been stored for at least 2 weeks, with daily changes of the medium. In the present study, we studied the multiplication rate of P. falciparum in cultures containing erythrocytes of various ages together with changing the medium at various intervals of time. Our results strongly suggest that the rate of in vitro multiplication of P. falciparum was higher in freshly collected erythrocytes than in aged erythrocytes regardless of the anticoagulant and that when the parasitemia is lower than 8% with a hematocrit of 5%, the medium change interval can be as long as 48 hr without a great reduction in the rate of multiplication. Copyright © The Korean Academy of Medical Sciences.
DOI
10.3346/jkms.2007.22.6.1022
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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