Chitosan film grafted with fusion protein of FGF-2 and fibronectin-FGF for tissue regeneration therapy

Abstract

The biological activity of protein-grafted chitosan was assayed by measuring the attachment of osteogenic cell, (HOS)(Human Osteogenic Sarcoma). It was shown that the acidity of the films was neutralized with 2mL of a solution. It was also shown that the Chitosan films were soaked in PBS buffer for 1h to hydrate the films. The Seeding of HOS onto the surface of the wells containing ungrafted and protein-grafted chitosan films resulted in seeding density of 7×104 cells/well.