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Construction of a garlic BAC library and chromosomal assignment of BAC clones using the FISH technique

Title
Construction of a garlic BAC library and chromosomal assignment of BAC clones using the FISH technique
Authors
Lee H.-R.Eom E.-M.Lim Y.-P.Bang J.-W.Lee D.-H.
Ewha Authors
이동희
SCOPUS Author ID
이동희scopus
Issue Date
2003
Journal Title
Genome
ISSN
0831-2796JCR Link
Citation
vol. 46, no. 3, pp. 514 - 520
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
For molecular and cytogenetic studies, two partial bacterial artificial chromosome (BAC) libraries of the garlic cultivar Allium sativum L. 'Danyang' were constructed using high molecular weight (HMW) garlic DNA, the pBAC1-SACB1 vector, and the pIndigoBAC536 vector. The average insert size of the BAC library was about 90 kb. The sequence compositions of the BAC clones were characterized by Southern hybridization with garlic genomic DNA and a repetitive sequence clone of garlic. Two BAC clones with weak signals (thus implying mostly unique sequences), GBC2-5e and GBC2-4d, were selected for FISH analysis. FISH analysis localized the GBC2-5e (∼100 kb) BAC clone on the long arm of garlic chromosome 7. The other BAC clone, GBC2-4d (∼110 kb), gave rise to discrete FISH signals on a mid-size early metaphase chromosome. The FISH screening with BAC clones proved to be a useful resource for molecular cytogenetic studies of garlic, and will be useful for further mapping and sequencing studies of important genes of this plant.
DOI
10.1139/g03-012
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일반대학원 > 바이오융합과학과 > Journal papers
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