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p-terphenyl curtisians protect cultured neuronal cells against glutamate neurotoxicity via iron chelation

Title
p-terphenyl curtisians protect cultured neuronal cells against glutamate neurotoxicity via iron chelation
Authors
Lee I.-K.Yun B.-S.Kim J.-P.Kim W.-G.Ryoo I.-J.Oh S.Kim Y.-H.Yoo I.-D.
Ewha Authors
오세관
SCOPUS Author ID
오세관scopus
Issue Date
2003
Journal Title
Planta Medica
ISSN
0032-0943JCR Link
Citation
vol. 69, no. 6, pp. 513 - 517
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The hyperactivity of ionotropic glutamate receptors has been implicated in the development of the neuronal cell death seen in many neurodegenerative processes including ischemic stroke, traumatic brain injury, and epilepsy. Thus neuronal protection against glutamate-induced neurotoxicity is considered as an appropriate therapeutic strategy for preventing and treating neurodegenerative diseases. Whilst searching for blockers of glutamate-induced toxicity in mouse cortical cells, we isolated p-terphenyl curtisians A - D from the mushroom Paxillus curtisii. Curtisians protected cortical neurons from glutamate-induced toxicity in a dose-dependent manner. Among the glutamate receptor subtypes, curtisians were found to block NMDA receptor-mediated but not AMPA/kainate-mediated cell death. In addition, we found that curtisians exhibited potent antioxidative activity against iron-mediated oxidative damage which was generated by H2O2 neurotoxocity and lipid peroxidation, but no activity was detected in the superoxide, DPPH and ABTS radical scavenging systems, and in protection of N18-RE-105 cells subjected to glutamate-induced glutathione depletion. This effect was likely due to the iron chelating properties of curtisians. The iron chelation ability of curtisians was then further investigated on DNA single strand breakage (SSB) induced by the addition of iron and H2O2, and curtisians prevented DNA SSB like the iron chelator desferrioxamine. These results suggest that the neuroprotective action of curtisians is dependent on their ability to chelate iron as well as to block the NMDA receptor, and that in this context curtisians may be useful as neuroprotective agents against neurological disorders which result in neuronal cell death.
DOI
10.1055/s-2003-40650
Appears in Collections:
의과대학 > 의학과 > Journal papers
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