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dc.contributor.author강상원-
dc.date.accessioned2016-08-28T11:08:40Z-
dc.date.available2016-08-28T11:08:40Z-
dc.date.issued2002-
dc.identifier.issn0021-9258-
dc.identifier.otherOAK-12621-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/228712-
dc.description.abstractBy following peroxiredoxin I (Prx I)-dependent NADPH oxidation spectrophotometrically, we observed that Prx I activity decreased gradually with time. The decay in activity was coincident with the conversion of Prx I to a more acidic species as assessed by two-dimensional gel electrophoresis. Mass spectral analysis and studies with Cys mutants determined that this shift in pI was due to selective oxidation of the catalytic site Cys51-SH to Cys51-SO2H. Thus, Cys51-SOH generated as an intermediate during catalysis appeared to undergo occasional further oxidation to Cys51-SO2H, which cannot be reversed by thioredoxin. The presence of H2O2 alone was not sufficient to cause oxidation of Cys51 to Cys51-SO2H. Rather, the presence of complete catalytic components (H2O2, thioredoxin, thioredoxin reductase, and NADPH) was necessary, indicating that such hyperoxidation occurs only when Prx I is engaged in the catalytic cycle. Likewise, hyperoxidation of Cys172/Ser172 mutant Prx I required not only H2O2, but also a catalysis-supporting thiol (dithiothreitol). Kinetic analysis of Prx I inactivation in the presence of a low steady-state level (<1 μm) of H2O2 indicated that Prx I was hyperoxidized at a rate of 0.072% per turnover at 30°C. Hyperoxidation of Prx I was also detected in HeLa cells treated with H2O2.-
dc.languageEnglish-
dc.titleInactivation of human peroxiredoxin I during catalysis as the result of the oxidation of the catalytic site cysteine to cysteine-sulfinic acid-
dc.typeArticle-
dc.relation.issue41-
dc.relation.volume277-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage38029-
dc.relation.lastpage38036-
dc.relation.journaltitleJournal of Biological Chemistry-
dc.identifier.doi10.1074/jbc.M206626200-
dc.identifier.scopusid2-s2.0-0037064080-
dc.author.googleYang K.-S.-
dc.author.googleKang S.W.-
dc.author.googleWoo H.A.-
dc.author.googleHwang S.C.-
dc.author.googleChae H.Z.-
dc.author.googleKim K.-
dc.author.googleRhee S.G.-
dc.contributor.scopusid강상원(55731433900)-
dc.date.modifydate20190901081003-


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