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Escherichia coli O157: H7 LPS O-side chains and pO157 are required for killing Caenorhabditis elegans

Escherichia coli O157: H7 LPS O-side chains and pO157 are required for killing Caenorhabditis elegans
Youn M.Lee K.-M.Kim S.H.Lim J.Yoon J.W.Park S.
Ewha Authors
Issue Date
Journal Title
Biochemical and Biophysical Research Communications
0006-291XJCR Link
Biochemical and Biophysical Research Communications vol. 436, no. 3, pp. 388 - 393
Document Type
As a model host, the nematode Caenorhabditis elegans has been used for studying unknown pathogen-host interactions and identifying novel virulence factors in bacterial pathogens. Among the bacterial pathogens that can induce death of C. elegans is enterohemorrhagic Escherichia coli (EHEC) O157:H7, a major serotype of EHEC that causes hemorrhagic colitis and hemolytic uremic syndrome in humans and animals. However, it is unknown which EHEC O157:H7 factors are required for nematode death. In this study, bacterial ability to kill C. elegans was tested for several EHEC O157:H7 wild-type and mutant strains missing one virulence-associated factor, including Shiga toxins, enterohemolysin, pO157 (a large virulence plasmid in EHEC O157:H7), Type 3 secretion system, LuxS, and lipopolysaccharide (LPS) O-side chains. Our results demonstrate that only mutants lacking either pO157 or LPS O-side chains cause full attenuation in killing C. elegans. The LPS O-side chain-defective δ. perA mutant strain was not able to colonize in the intestine even at 24. h post-feeding with C. elegans, while the wild-type strain began to accumulate and colonize in the intestine as early as 3. h post-feeding. A simple complementation of the mutant strain with the plasmid carrying the intact perA gene in trans completely restored the production of LPS O-side chains, as well as the ability to kill C. elegans. Our results show that pO157 and PerA are required for EHEC O157:H7 to kill C. elegans. © 2013 Elsevier Inc.
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