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Ghrelin receptor is activated by naringin and naringenin, constituents of a prokinetic agent Poncirus fructus

Title
Ghrelin receptor is activated by naringin and naringenin, constituents of a prokinetic agent Poncirus fructus
Authors
Jang Y.Kim S.-W.Oh J.Hong G.-S.Seo E.-K.Oh U.Shim W.-S.
Ewha Authors
서은경
SCOPUS Author ID
서은경scopus
Issue Date
2013
Journal Title
Journal of Ethnopharmacology
ISSN
0378-8741JCR Link
Citation
vol. 148, no. 2, pp. 459 - 465
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Ethnopharmacological relevance Poncirus fructus (PF), also known as a dried immature fruit of Poncirus trifoliata (L.) Raf. (Rutaceae), has long been traditionally used for the various gastrointestinal disorders in Eastern Asia. Aim of study The aqueous extract of PF (PF-W) has the strong prokinetic effect, yet the underlying mechanism is still elusive. The present study investigated whether PF-W has any effect on motilin receptor or ghrelin receptor, since these receptors enhance intestinal motility when activated. Materials and methods The effect of PF-W and its components on motilin or ghrelin receptor was determined by calcium imaging and whole-cell patch clamp methods. Results PF-W activates the ghrelin receptor, but not the motilin receptor, resulting in a transient increase of intracellular calcium levels. Furthermore, among various constituents of PF, only naringin and naringenin evoked the intracellular calcium augmentation via the ghrelin receptor. Moreover, cortistatin-8 - a ghrelin receptor inhibitor - specifically blocked naringin- and naringenin-induced calcium increases. In addition, naringin and naringenin induced inward currents in ghrelin receptor-expressing cells under whole-cell patch clamp configuration. Conclusion PF-W activates the ghrelin receptor, and naringin and naringenin are key constituents responsible for the activation of ghrelin receptor. Therefore, the present study suggests that the ghrelin receptor is a molecular entity responsible for the strong prokinetic activity of PF-W. © 2013 Elsevier Ireland Ltd. All rights reserved.
DOI
10.1016/j.jep.2013.04.039
Appears in Collections:
약학대학 > 약학과 > Journal papers
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