Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김희선 | * |
dc.date.accessioned | 2016-08-28T10:08:39Z | - |
dc.date.available | 2016-08-28T10:08:39Z | - |
dc.date.issued | 2013 | * |
dc.identifier.issn | 1021-335X | * |
dc.identifier.other | OAK-9891 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223550 | - |
dc.description.abstract | Previously, we reported that 20-O-(β-D-glucopyranosyl)- 20(S)-protopanaxadiol (Compound K, a metabolite of ginseng saponin) induces mitochondria-dependent and caspase-dependent apoptosis in HT-29 human colon cancer cells via the generation of reactive oxygen species. The aim of the present study was to elucidate the mechanism underlying apoptosis induced by Compound K with respect to endoplasmic reticulum (ER) stress in HT-29 cells. In the present study, Compound K induced apoptotic cell death as confirmed by DNA fragmentation and apoptotic sub-G1 cell population. Compound K also induced ER stress as indicated by staining with ER tracker, cytosolic and mitochondrial Ca2+ overloading, phosphorylation of protein-kinase-like endoplasmic reticulum kinase (PERK), phosphorylation of eukaryotic initiation factor-2a (eIF-2a), phosphorylation of IRE-1, splicing of ER stress-specific X-box transcription factor-1 (XBP-1), cleavage of activating transcription factor-6 (ATF-6), upregulation of glucose-regulated protein-78 (GRP-78/BiP) and CCAAT/enhancer-binding protein-homologous protein (CHOP), and cleavage of caspase-12. Furthermore, downregulation of CHOP expression using siCHOP RNA attenuated Compound K-induced apoptosis. Taken together, these results support the important role of ER stress response in mediating Compound K-induced apoptosis in human colon cancer cells. © 2013 Spandidos Publications Ltd. All rights reserved. | * |
dc.language | English | * |
dc.title | 20-O-(β-D-glucopyranosyl)-20(S)-protopanaxadiol induces apoptosis via induction of endoplasmic reticulum stress in human colon cancer cells | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 29 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1365 | * |
dc.relation.lastpage | 1370 | * |
dc.relation.journaltitle | Oncology Reports | * |
dc.identifier.doi | 10.3892/or.2013.2270 | * |
dc.identifier.wosid | WOS:000316510600014 | * |
dc.identifier.scopusid | 2-s2.0-84874752073 | * |
dc.author.google | Zhang R. | * |
dc.author.google | Chung Y. | * |
dc.author.google | Kim H.S. | * |
dc.author.google | Kim D.-H. | * |
dc.author.google | Chang W.Y. | * |
dc.author.google | Hyun J.W. | * |
dc.contributor.scopusid | 김희선(57191372551) | * |
dc.date.modifydate | 20240118140922 | * |