View : 37 Download: 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author장준-
dc.date.accessioned2017-08-28T23:34:44Z-
dc.date.available2017-08-28T23:34:44Z-
dc.date.issued2012-
dc.identifier.issn0041-1337-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/223248-
dc.description.abstractBACKGROUND: In many humanized mouse models, there are few T cells in the engrafted human cell, whereas the number of B cells is high. We attempted to overcome this limitation and investigate whether the entire process of human T cell development arose similarly to the process in humans, as previously reported. METHODS: To produce an advanced humanized mice model, we transplanted human fetal liver/thymus tissue subrenally and injected human CD34+ stem cells intravenously into NOD/SCID/IL2Rgamma null (NSG) mice. RESULTS: Humanized mice transplanted with fetal thymus/liver tissues and fetal liver-derived CD34+ stem cells (FLT+FLCD34) showed higher levels of human cells and T cells than mice transplanted with fetal liver-derived CD34+ stem cells only (FLCD34). In the transplanted thymus tissue of FLT+FLCD34 mice, thymus seeding progenitors (TSPs), early thymic progenitors (ETPs), pre-T cells, and all the other human T cell populations were identified. In the periphery, FLT+FLCD34 mice have high levels of CD45RA+ T cells; conversely, FLCD34 mice have higher levels of CD45RO+ T cells. The CD45RO+ T cells of FLCD34 mice proliferated rapidly after stimulation and exhibited innate T cells properties, expressing PLZF (promyelocytic leukemia zinc finger protein). CONCLUSION: Human T cells educated by mouse MHC II in mice without a human thymus differ from normal human T cells. On the basis of these findings, numerous T cell-tropic human diseases could be explored in our humanized mice and molecular aspects of human T cell development could be also studied extensively. Copyright © 2012 Lippincott Williams &Wilkins.-
dc.languageEnglish-
dc.titleSystemic human T cell developmental processes in humanized mice cotransplanted with human fetal thymus/liver tissue and hematopoietic stem cells-
dc.typeArticle-
dc.relation.issue11-
dc.relation.volume94-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage1095-
dc.relation.lastpage1102-
dc.relation.journaltitleTransplantation-
dc.identifier.doi10.1097/TP.0b013e318270f392-
dc.identifier.wosidWOS:000312072600010-
dc.identifier.scopusid2-s2.0-84871531019-
dc.author.googleJoo S.-Y.-
dc.author.googleChung Y.S.-
dc.author.googleChoi B.-
dc.author.googleKim M.-
dc.author.googleKim J.-H.-
dc.author.googleJun T.-G.-
dc.author.googleChang J.-
dc.author.googleSprent J.-
dc.author.googleSurh C.D.-
dc.author.googleJoh J.-W.-
dc.author.googleKim S.J.-
dc.contributor.scopusid장준(8735999100)-
dc.date.modifydate20180104081001-
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE