Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 장준 | * |
dc.date.accessioned | 2016-08-28T10:08:08Z | - |
dc.date.available | 2016-08-28T10:08:08Z | - |
dc.date.issued | 2012 | * |
dc.identifier.issn | 0041-1337 | * |
dc.identifier.other | OAK-9534 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223248 | - |
dc.description.abstract | BACKGROUND: In many humanized mouse models, there are few T cells in the engrafted human cell, whereas the number of B cells is high. We attempted to overcome this limitation and investigate whether the entire process of human T cell development arose similarly to the process in humans, as previously reported. METHODS: To produce an advanced humanized mice model, we transplanted human fetal liver/thymus tissue subrenally and injected human CD34+ stem cells intravenously into NOD/SCID/IL2Rgamma null (NSG) mice. RESULTS: Humanized mice transplanted with fetal thymus/liver tissues and fetal liver-derived CD34+ stem cells (FLT+FLCD34) showed higher levels of human cells and T cells than mice transplanted with fetal liver-derived CD34+ stem cells only (FLCD34). In the transplanted thymus tissue of FLT+FLCD34 mice, thymus seeding progenitors (TSPs), early thymic progenitors (ETPs), pre-T cells, and all the other human T cell populations were identified. In the periphery, FLT+FLCD34 mice have high levels of CD45RA+ T cells; conversely, FLCD34 mice have higher levels of CD45RO+ T cells. The CD45RO+ T cells of FLCD34 mice proliferated rapidly after stimulation and exhibited innate T cells properties, expressing PLZF (promyelocytic leukemia zinc finger protein). CONCLUSION: Human T cells educated by mouse MHC II in mice without a human thymus differ from normal human T cells. On the basis of these findings, numerous T cell-tropic human diseases could be explored in our humanized mice and molecular aspects of human T cell development could be also studied extensively. Copyright © 2012 Lippincott Williams &Wilkins. | * |
dc.language | English | * |
dc.title | Systemic human T cell developmental processes in humanized mice cotransplanted with human fetal thymus/liver tissue and hematopoietic stem cells | * |
dc.type | Article | * |
dc.relation.issue | 11 | * |
dc.relation.volume | 94 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1095 | * |
dc.relation.lastpage | 1102 | * |
dc.relation.journaltitle | Transplantation | * |
dc.identifier.doi | 10.1097/TP.0b013e318270f392 | * |
dc.identifier.wosid | WOS:000312072600010 | * |
dc.identifier.scopusid | 2-s2.0-84871531019 | * |
dc.author.google | Joo S.-Y. | * |
dc.author.google | Chung Y.S. | * |
dc.author.google | Choi B. | * |
dc.author.google | Kim M. | * |
dc.author.google | Kim J.-H. | * |
dc.author.google | Jun T.-G. | * |
dc.author.google | Chang J. | * |
dc.author.google | Sprent J. | * |
dc.author.google | Surh C.D. | * |
dc.author.google | Joh J.-W. | * |
dc.author.google | Kim S.J. | * |
dc.contributor.scopusid | 장준(8735999100) | * |
dc.date.modifydate | 20231120165756 | * |