View : 60 Download: 0

Apoptotic cell instillation after bleomycin attenuates lung injury through hepatocyte growth factor induction

Title
Apoptotic cell instillation after bleomycin attenuates lung injury through hepatocyte growth factor induction
Authors
Lee Y.-J.Moon C.Lee S.H.Park H.-J.Seoh J.-Y.Cho M.-S.Kang J.L.
Ewha Authors
서주영이지희조민선
SCOPUS Author ID
서주영scopus; 이지희scopus; 조민선scopus
Issue Date
2012
Journal Title
European Respiratory Journal
ISSN
0903-1936JCR Link
Citation
vol. 40, no. 2, pp. 424 - 435
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Apoptotic cell clearance by macrophages and neighbouring tissue cells induces hepatocyte growth factor (HGF) secretion. HGF plays a key role in alveolar epithelial regeneration and reconstruction after lung injury. Direct in vivo exposure to apoptotic cells enhances HGF production, resulting in attenuation of pulmonary injury. We investigated the direct effect of in vivo exposure to apoptotic cells in bleomycin-stimulated lungs (2 days old) on HGF induction. Furthermore, sequential changes of bleomycin-induced HGF production following apoptotic cell instillation related to the changes in inflammatory and fibrotic responses were assessed. At 2 h after apoptotic cell instillation into bleomycin-stimulated lungs, the levels of HGF mRNA and protein production, and apoptotic cell clearance by alveolar macrophages were enhanced. Furthermore, HGF induction persistently increased following apoptotic cell instillation up to 21 days after bleomycin treatment. Apoptotic cell instillation attenuated bleomycin-induced proinflammatory mediator production, inflammatory cell recruitment and total protein levels. Apoptotic cell instillation also induced antiapoptotic and antifibrotic effects. These antiinflammatory and antiapoptotic effects could be reversed by co-administration of HGFneutralising antibody. These findings indicate that in vivo exposure to apoptotic cells enhances transcriptional HGF production in bleomycin-stimulated lungs, resulting in attenuation of lung injury and fibrosis. Copyright©ERS 2012.
DOI
10.1183/09031936.00096711
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE