Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이지희 | * |
dc.contributor.author | 조민선 | * |
dc.date.accessioned | 2016-08-28T10:08:36Z | - |
dc.date.available | 2016-08-28T10:08:36Z | - |
dc.date.issued | 2012 | * |
dc.identifier.issn | 0041-008X | * |
dc.identifier.other | OAK-9087 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/222906 | - |
dc.description.abstract | Mer receptor tyrosine kinase (Mer) regulates macrophage activation and promotes apoptotic cell clearance. Mer activation is regulated through proteolytic cleavage of the extracellular domain. To determine if membrane-bound Mer is cleaved during bleomycin-induced lung injury, and, if so, how preventing the cleavage of Mer enhances apoptotic cell uptake and down-regulates pulmonary immune responses. During bleomycin-induced acute lung injury in mice, membrane-bound Mer expression decreased, but production of soluble Mer and activity as well as expression of disintegrin and metalloproteinase 17 (ADAM17) were enhanced Treatment with the ADAM inhibitor TAPI-0 restored Mer expression and diminished soluble Mer production. Furthermore, TAPI-0 increased Mer activation in alveolar macrophages and lung tissue resulting in enhanced apoptotic cell clearance in vivo and ex vivo by alveolar macrophages. Suppression of bleomycin-induced pro-inflammatory mediators, but enhancement of hepatocyte growth factor induction were seen after TAPI-0 treatment. Additional bleomycin-induced inflammatory responses reduced by TAPI-0 treatment included inflammatory cell recruitment into the lungs, levels of total protein and lactate dehydrogenase activity in bronchoalveolar lavage fluid, as well as caspase-3 and caspase-9 activity and alveolar epithelial cell apoptosis in lung tissue. Importantly, the effects of TAPI-0 on bleomycin-induced inflammation and apoptosis were reversed by coadministration of specific Mer-neutralizing antibodies. These findings suggest that restored membrane-bound Mer expression by TAPI-0 treatment may help resolve lung inflammation and apoptosis after bleomycin treatment. © 2012 Elsevier Inc. | * |
dc.language | English | * |
dc.title | Preventing cleavage of Mer promotes efferocytosis and suppresses acute lung injury in bleomycin treated mice | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 263 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 61 | * |
dc.relation.lastpage | 72 | * |
dc.relation.journaltitle | Toxicology and Applied Pharmacology | * |
dc.identifier.doi | 10.1016/j.taap.2012.05.024 | * |
dc.identifier.wosid | WOS:000307208900009 | * |
dc.identifier.scopusid | 2-s2.0-84864135401 | * |
dc.author.google | Lee Y.-J. | * |
dc.author.google | Lee S.-H. | * |
dc.author.google | Youn Y.-S. | * |
dc.author.google | Choi J.-Y. | * |
dc.author.google | Song K.-S. | * |
dc.author.google | Cho M.-S. | * |
dc.author.google | Kang J.L. | * |
dc.contributor.scopusid | 이지희(7404517577) | * |
dc.contributor.scopusid | 조민선(13205279200) | * |
dc.date.modifydate | 20240118154227 | * |