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Cd4 +cd25 + regulatory t cells from MRL/lpr mice were functionally more active in vitro but did not prevent spontaneous as well as adriamycin-induced nephropathy in vivo

Title
Cd4 +cd25 + regulatory t cells from MRL/lpr mice were functionally more active in vitro but did not prevent spontaneous as well as adriamycin-induced nephropathy in vivo
Authors
Kim H.-R.Kie J.-H.Lim W.Moon B.-I.Kim S.C.Seoh J.-Y.
Ewha Authors
서주영김승철문병인임우성김형란
SCOPUS Author ID
서주영scopus; 김승철scopus; 문병인scopus; 임우성scopus; 김형란scopus
Issue Date
2012
Journal Title
Rheumatology (United Kingdom)
ISSN
1462-0324JCR Link
Citation
vol. 51, no. 8, pp. 1357 - 1367
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Objective. The frequency and function of Tregs are important in the pathogenesis of SLE. Nonetheless, the function of Tregs is still controversial in SLE patients and lupus mouse models. In the present study, we investigated the suppressive function of Tregs from MRL/lpr mice in vitro and in vivo by using an alternative quantitative assay.Methods. We assessed the suppressive function of CD4 +CD25 + Tregs, the proliferative activity of CD4 +CD25 - effector T cells (Teffs) and the feeder activity of CD11c + dendritic cells (DCs), isolated from the spleens of MRL/lpr mice and wild-type (WT) MRL/+ mice, by carboxyfluorescein diacetate succinimidyl ester dilution assay stimulated with two distinct types of signals, weak and strong. In order to assess the protective function of Tregs from an immune-mediated disease in vivo, we induced renal damage by injecting adriamycin (ADN) into the mice.Results. The in vitro assay showed enhanced suppressive activity of Tregs and feeder activity of DCs, but far less proliferative activity of Teffs from MRL/lpr mice, compared with those from the WT mice. The in vivo study showed more severe ADN-induced nephropathy in MRL/lpr mice than in the WT mice, while mild interstitial nephritis had already begun spontaneously by 16 weeks in MRL/lpr mice.Conclusion. It was suggested that Tregs from MRL/lpr mice were functionally competent and intrinsically more active in vitro, but they were not capable of preventing the ADN-induced as well as the spontaneously developing nephropathy in vivo. © The Author 2012. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved.
DOI
10.1093/rheumatology/kes058
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의과대학 > 의학과 > Journal papers
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