Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이지희 | * |
dc.contributor.author | 정영해 | * |
dc.date.accessioned | 2016-08-28T10:08:28Z | - |
dc.date.available | 2016-08-28T10:08:28Z | - |
dc.date.issued | 2012 | * |
dc.identifier.issn | 0360-4012 | * |
dc.identifier.other | OAK-8997 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/222828 | - |
dc.description.abstract | Human immunodeficiency virus (HIV)-induced inflammation, and its consequences within the central nervous system (CNS), must be countered by multiple pharmacologic agents, and 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) may hold promise in the treatment of pathologies associated with this inflammatory response. 15d-PGJ2 can repress the inflammatory response by means of peroxisome proliferator-activated receptor-γ (PPARγ)-dependent and -independent mechanisms. However, its precise role and antiinflammatory mechanism in the hippocampus remain poorly understood. In the present study, rat hippocampal slices were stimulated with full-length HIV-1 Tat protein to investigate the role of 15d-PGJ2 8in the hippocampal inflammatory response. Pretreatment of slices with 15d-PGJ2 markedly reduced Tat-induced monocyte chemoattractant protein-1 (MCP-1/CCL2) production. Interestingly, the PPARγ antagonist GW9662 did not inhibit action of 15d-PGJ2, confirming the latter's PPARγ-independent mechanism of mediating antiinflammatory effects. Despite 15d-PGJ2's increasing the expression of heme oxygenase-1 (HO-1), its action was not abrogated by the HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX), nor was it recapitulated by HO-1 inducers such as cobalt protoporphyrin (CoPP). Moreover, short interfering RNA (siRNA)-directed knockdown of HO-1 did not abolish the antiinflammatory action of 15d-PGJ2 against Tat-induced MCP-1 production in human microglia-like THP-1 cells. Conversely, 15d-PGJ2 suppressed Tat-induced ERK1/2 activation, decreasing MCP-1 production upon Tat stimulation. The NADPH oxidase inhibitors DPI and apocynin also abrogated Tat-stimulated ERK1/2 activation, reducing MCP-1 production. Collectively, these data demonstrate that the antiinflammatory effects of 15d-PGJ2 on the hippocampus are exerted through inhibition of Tat-mediated ERK1/2 activation, coupled with that of a redox-sensitive pathway, independent of PPARγ and HO-1. © 2012 Wiley Periodicals, Inc. | * |
dc.language | English | * |
dc.title | 15-deoxy-Δ12,14-prostaglandin J2 inhibits human immunodeficiency virus-1 tat-induced monocyte chemoattractant protein-1/CCL2 production by blocking the extracellular signal-regulated kinase-1/2 signaling pathway independently of peroxisome proliferator-activated receptor-γ and heme oxygenase-1 in rat hippocampal slices | * |
dc.type | Article | * |
dc.relation.issue | 9 | * |
dc.relation.volume | 90 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1732 | * |
dc.relation.lastpage | 1742 | * |
dc.relation.journaltitle | Journal of Neuroscience Research | * |
dc.identifier.doi | 10.1002/jnr.23051 | * |
dc.identifier.wosid | WOS:000306272100006 | * |
dc.identifier.scopusid | 2-s2.0-84863776881 | * |
dc.author.google | Kim S.E. | * |
dc.author.google | Lee E.O. | * |
dc.author.google | Yang J.H. | * |
dc.author.google | Kang J.H.L. | * |
dc.author.google | Suh Y.-H. | * |
dc.author.google | Chong Y.H. | * |
dc.contributor.scopusid | 이지희(7404517577) | * |
dc.contributor.scopusid | 정영해(7201371824) | * |
dc.date.modifydate | 20240116125728 | * |