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Changes of gene expression after bone marrow cell transfusion in rats with monocrotaline-induced pulmonary hypertension

Title
Changes of gene expression after bone marrow cell transfusion in rats with monocrotaline-induced pulmonary hypertension
Authors
Kim K.C.Lee H.R.Kim S.J.Cho M.-S.Hong Y.M.
Ewha Authors
홍영미조민선김관창
SCOPUS Author ID
홍영미scopus; 조민선scopus; 김관창scopus
Issue Date
2012
Journal Title
Journal of Korean Medical Science
ISSN
1011-8934JCR Link
Citation
vol. 27, no. 6, pp. 605 - 613
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Pulmonary artery hypertension (PAH) causes right ventricular failure and possibly even death by a progressive increase in pulmonary vascular resistance. Bone marrow-derived mesenchymal stem cell therapy has provided an alternative treatment for ailments of various organs by promoting cell regeneration at the site of pathology. The purpose of this study was to investigate changes of pulmonary haemodynamics, pathology and expressions of various genes, including ET (endothelin)-1, ET receptor A (ERA), endothelial nitric oxide synthase (NOS) 3, matrix metalloproteinase (MMP) 2, tissue inhibitor of matrix metalloproteinase (TIMP), interleukin (IL)-6 and tumor necrosis factor (TNF)-α in monocrotaline (MCT)-induced PAH rat models after bone marrow cell (BMC) transfusion. The rats were grouped as the control (C) group, monocrotaline (M) group, and BMC transfusion (B) group. M and B groups received subcutaneous (sc) injection of MCT (60 mg/kg). BMCs were transfused by intravenous injection at the tail 1 week after MCT injection in B group. Results showed that the average RV pressure significantly decreased in the B group compared with the M group. RV weight and the ratio of RH/LH+septum significantly decreased in the B group compared to the M group. Gene expressions of ET-1, ERA, NOS 3, MMP 2, TIMP, IL-6, and TNF-α significantly decreased in week 4 in the B group compared with the M group. In conclusion, BMC transfusion appears to improve survival rate, RVH, and mean RV pressure, and decreases gene expressions of ET-1, ERA, NOS 3, MMP 2, TIMP, IL-6, and TNF-α. © 2012 The Korean Academy of Medical Sciences.
DOI
10.3346/jkms.2012.27.6.605
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의과대학 > 의학과 > Journal papers
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