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Comparison of the AdvanSure human papillomavirus screening real-time PCR, the abbott RealTime high risk human papillomavirus test, and the hybrid capture human papillomavirus DNA test for the detection of human papillomavirus
- Comparison of the AdvanSure human papillomavirus screening real-time PCR, the abbott RealTime high risk human papillomavirus test, and the hybrid capture human papillomavirus DNA test for the detection of human papillomavirus
- Hwang Y.; Lee M.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Annals of Laboratory Medicine
- vol. 32, no. 3, pp. 201 - 205
- SCIE; SCOPUS; KCI
- Background: We evaluated the performance of various commercial assays for the molecular detection of human papillomavirus (HPV); the recently developed AdvanSure HPV Screening real-time PCR assay (AdvanSure PCR) and the Abbott RealTime High Risk HPV PCR assay (Abbott PCR) were compared with the Hybrid Capture 2 HPV DNA Test (HC2). Methods: All 3 tests were performed on 177 samples, and any sample that showed a discrepancy in any of the 3 tests was genotyped using INNO-LiPA HPV genotyping and/or sequencing. On the basis of these results, we obtained a consensus HPV result, and the performance of each test was evaluated. We also evaluated high-risk HPV 16/18 detection by using the 2 real-time PCR assays. Results: Among the 177 samples, 65 were negative and 75 were positive in all 3 assays; however, the results of the 3 assays with 37 samples were discrepant. Compared with the consensus HPV result, the sensitivities and specificities of HC2, AdvanSure PCR, and Abbott PCR were 97.6%, 91.7%, and 86.9% and 83.9%, 98.8%, and 100.0%, respectively. For HPV type 16/18 detection, the concordance rate between the AdvanSure PCR and Abbott PCR assays was 98.3%; however, 3 samples were discrepant (positive in AdvanSure PCR and negative in Abbott PCR) and were confirmed as HPV type 16 by INNO-LiPA genotyping and/or sequencing. Conclusions: For HPV detection, the AdvanSure HPV Screening real-time PCR assay and the Abbott PCR assay are less sensitive but more specific than the HC2 assay, but can simultaneously differentiate type 16/18 HPV from other types. © The Korean Society for Laboratory Medicine.
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