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Comparison of the AdvanSure human papillomavirus screening real-time PCR, the abbott RealTime high risk human papillomavirus test, and the hybrid capture human papillomavirus DNA test for the detection of human papillomavirus

Title
Comparison of the AdvanSure human papillomavirus screening real-time PCR, the abbott RealTime high risk human papillomavirus test, and the hybrid capture human papillomavirus DNA test for the detection of human papillomavirus
Authors
Hwang Y.Lee M.
Ewha Authors
이미애
SCOPUS Author ID
이미애scopus
Issue Date
2012
Journal Title
Annals of Laboratory Medicine
ISSN
2234-3806JCR Link
Citation
vol. 32, no. 3, pp. 201 - 205
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Background: We evaluated the performance of various commercial assays for the molecular detection of human papillomavirus (HPV); the recently developed AdvanSure HPV Screening real-time PCR assay (AdvanSure PCR) and the Abbott RealTime High Risk HPV PCR assay (Abbott PCR) were compared with the Hybrid Capture 2 HPV DNA Test (HC2). Methods: All 3 tests were performed on 177 samples, and any sample that showed a discrepancy in any of the 3 tests was genotyped using INNO-LiPA HPV genotyping and/or sequencing. On the basis of these results, we obtained a consensus HPV result, and the performance of each test was evaluated. We also evaluated high-risk HPV 16/18 detection by using the 2 real-time PCR assays. Results: Among the 177 samples, 65 were negative and 75 were positive in all 3 assays; however, the results of the 3 assays with 37 samples were discrepant. Compared with the consensus HPV result, the sensitivities and specificities of HC2, AdvanSure PCR, and Abbott PCR were 97.6%, 91.7%, and 86.9% and 83.9%, 98.8%, and 100.0%, respectively. For HPV type 16/18 detection, the concordance rate between the AdvanSure PCR and Abbott PCR assays was 98.3%; however, 3 samples were discrepant (positive in AdvanSure PCR and negative in Abbott PCR) and were confirmed as HPV type 16 by INNO-LiPA genotyping and/or sequencing. Conclusions: For HPV detection, the AdvanSure HPV Screening real-time PCR assay and the Abbott PCR assay are less sensitive but more specific than the HC2 assay, but can simultaneously differentiate type 16/18 HPV from other types. © The Korean Society for Laboratory Medicine.
DOI
10.3343/alm.2012.32.3.201
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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