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Soluble HSPB1 regulates VEGF-mediated angiogenesis through their direct interaction

Title
Soluble HSPB1 regulates VEGF-mediated angiogenesis through their direct interaction
Authors
Lee Y.-J.Lee H.-J.Choi S.-H.Jin Y.B.An H.J.Kang J.-H.Yoon S.S.Lee Y.-S.
Ewha Authors
이윤실
SCOPUS Author ID
이윤실scopus
Issue Date
2012
Journal Title
Angiogenesis
ISSN
0969-6970JCR Link
Citation
vol. 15, no. 2, pp. 229 - 242
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Endothelial cell function is critical for angiogenic balance in both physiological and pathological conditions, such as wound healing and cancer, respectively. We report here that soluble heat shock protein beta-1 (HSPB1) is released primarily from endothelial cells (ECs), and plays a key role in regulating angiogenic balance via direct interaction with vascular endothelial growth factor (VEGF). VEGF-mediated phosphorylation of intracellular HSPB1 inhibited the secretion of HSPB1 and their binding activity in ECs. Interestingly, co-culture of tumor ECs with tumor cells decreased HSPB1 secretion from tumor ECs, suggesting that inhibition of HSPB1 secretion allows VEGF to promote angiogenesis. Additionally, neutralization of HSPB1 in a primary mouse sarcoma model promoted tumor growth, indicating the anti-angiogenic role of soluble HSPB1. Overexpression of HSPB1 by HSPB1 adenovirus was sufficient to suppress lung metastases of CT26 colon carcinoma in vivo, while neutralization of HSPB1 promoted in vivo wound healing. While VEGFinduced regulation of angiogenesis has been studied extensively, these findings illustrate the key contribution of HSPB1-VEGF interactions in the balance between physiological and pathological angiogenesis. © Springer Science+Business Media B.V. 2012.
DOI
10.1007/s10456-012-9255-3
Appears in Collections:
약학대학 > 약학과 > Journal papers
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