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Cell therapy for skin wound using fibroblast encapsulated poly(ethylene glycol)-poly(L-alanine) thermogel

Title
Cell therapy for skin wound using fibroblast encapsulated poly(ethylene glycol)-poly(L-alanine) thermogel
Authors
Yun E.J.Yon B.Joo M.K.Jeong B.
Ewha Authors
정병문
SCOPUS Author ID
정병문scopus
Issue Date
2012
Journal Title
Biomacromolecules
ISSN
1525-7797JCR Link
Citation
vol. 13, no. 4, pp. 1106 - 1111
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
As a new application of a thermogel, a poly- (ethylene glycol)-b-poly(L- alanine) (PEG-L-PA) gel encapsulating fibroblasts was investigated for wound healing. The fibroblasts were encapsulated by the temperature sensitive sol-to-gel transition of the polymer aqueous solution. Under the in vitro three-dimensional (3D) cell culture condition, the PEG-L-PA thermogel was comparable with Matrigel for cell proliferation and was significantly better than Matrigel for collagen types I and III formation. After confirming the excellent 3D microenvironment of the PEG-L-PA thermogel for fibroblasts, in vivo wound healing was investigated by injecting the cell-suspended polymer aqueous solution on incisions of rat skin, where the cell-encapsulated gel was formed in situ. Compared with the phosphate buffered saline treated system and the cell-free PEG-L-PA thermogel, the cell-encapsulated PEG-L-PA thermogel not only accelerated the wound closure but also improved epithelialization and the formation of skin appendages such as keratinocyte layer (epidermis), hair follicles, and sebaceous glands. The results demonstrate the potential of thermogels for cell therapy as an injectable tissue-engineering scaffold. © 2012 American Chemical Society.
DOI
10.1021/bm2018596
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자연과학대학 > 화학·나노과학전공 > Journal papers
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