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Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits

Title
Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits
Authors
Oh C.S.Lee S.J.Park J.B.Lee S.D.Seo S.B.Kim H.Y.Kim J.Kim Y.-S.Shin D.H.
Ewha Authors
김이석
SCOPUS Author ID
김이석scopus
Issue Date
2012
Journal Title
Journal of Forensic Sciences
ISSN
0022-1198JCR Link
Citation
vol. 57, no. 3, pp. 820 - 825
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR ® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n=8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR ® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples. © 2012 American Academy of Forensic Sciences.
DOI
10.1111/j.1556-4029.2011.02044.x
Appears in Collections:
의과대학 > 의학과 > Journal papers
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