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Production of a conjugated fatty acid by Bifidobacterium breve LMC520 from α-linolenic acid: Conjugated linolenic acid (CLnA)

Title
Production of a conjugated fatty acid by Bifidobacterium breve LMC520 from α-linolenic acid: Conjugated linolenic acid (CLnA)
Authors
Park H.G.Cho H.T.Song M.-C.Kim S.B.Kwon E.G.Choi N.J.Kim Y.J.
Ewha Authors
송명종
SCOPUS Author ID
송명종scopus
Issue Date
2012
Journal Title
Journal of Agricultural and Food Chemistry
ISSN
0021-8561JCR Link
Citation
vol. 60, no. 12, pp. 3204 - 3210
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
This study was performed to characterize natural CLnA isomer production by Bifidobacterium breve LMC520 of human origin in comparison to conjugated linoleic acid (CLA) production. B. breve LMC520 was found to be highly active in terms of CLnA production, of which the major portion was identified as cis-9,trans-11,cis-15 CLnA isomer by GC-MS and NMR analysis. B. breve LMC520 was incubated for 48 h using MRS medium (containing 0.05% l-cysteine•HCl) under different environmental conditions such as atmosphere, pH, and substrate concentration. The high conversion rate of α-linolenic acid (α-LNA) to CLnA (99%) was retained up to 2 mM α-LNA, and the production was proportionally increased nearly 7-fold with 8 mM by the 6 h of incubation under anaerobic conditions at a wide range of pH values (between 5 and 9). When α-LNA was compared with linoleic acid (LA) as a substrate for isomerization by B. breve LMC520, the conversion of α-LNA was higher than that of LA. These results demonstrated that specific CLnA isomer could be produced through active bacterial conversion at an optimized condition. Because many conjugated octadecatrienoic acids in nature are shown to play many positive roles, the noble isomer found in this study has potential as a functional source. © 2012 American Chemical Society.
DOI
10.1021/jf2041559
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연구기관 > 나노·바이오기술연구소 > Journal papers
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