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Dynamic immune cell accumulation during flow-induced atherogenesis in mouse carotid artery an expanded flow cytometry method

Title
Dynamic immune cell accumulation during flow-induced atherogenesis in mouse carotid artery an expanded flow cytometry method
Authors
Alberts-Grill N.Rezvan A.Son D.J.Qiu H.Kim C.W.Kemp M.L.Weyand C.M.Jo H.
Ewha Authors
조한중
Issue Date
2012
Journal Title
Arteriosclerosis, Thrombosis, and Vascular Biology
ISSN
1079-5642JCR Link
Citation
vol. 32, no. 3, pp. 623 - 632
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Objective-Inflammation plays a central role in atherosclerosis. However, the detailed changes in the composition and quantity of leukocytes in the arterial wall during atherogenesis are not fully understood in part because of the lack of suitable methods and animal models. Methods and Results-We developed a 10-fluorochrome, 13-parameter flow cytometry method to quantitate 7 major leukocyte subsets in a single digested arterial wall sample. Apolipoprotein E-deficient mice underwent left carotid artery (LCA) partial ligation and were fed a high-fat diet for 4 to 28 days. Monocyte/macrophages, dendritic cells, granulocytes, natural killer cells, and CD4 T cells significantly infiltrated the LCA as early as 4 days. Monocyte/macrophages and dendritic cells decreased between 7 and 14 days, whereas T-cell numbers remained steady. Leukocyte numbers peaked at 7 days, preceding atheroma formation at 14 days. B cells entered LCA by 14 days. Control right carotid and sham-ligated LCAs showed no significant infiltrates. Polymerase chain reaction and ELISA arrays showed that expression of proinflammatory cytokines and chemokines peaked at 7 and 14 days postligation, respectively. Conclusion-This is the first quantitative description of leukocyte number and composition over the life span of murine atherosclerosis. These results show that disturbed flow induces rapid and dynamic leukocyte accumulation in the arterial wall during the initiation and progression of atherosclerosis. © 2012 American Heart Association, Inc.
DOI
10.1161/ATVBAHA.111.242180
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일반대학원 > 바이오융합과학과 > Journal papers
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